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EPO CLIA Kit

EPO Reactivity: Mouse Chemiluminescent Sandwich ELISA 1.37 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Catalog No. ABIN5653972
  • Target See all EPO CLIA Kits
    EPO (Erythropoietin (EPO))
    Reactivity
    • 6
    • 6
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Mouse
    Detection Method
    Chemiluminescent
    Method Type
    Sandwich ELISA
    Detection Range
    1.37 pg/mL - 1000 pg/mL
    Minimum Detection Limit
    1.37 pg/mL
    Application
    ELISA
    Sample Type
    Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Erythropoietin (EPO). No significant cross-reactivity or interference between Erythropoietin (EPO) and analogues was observed.
    Sensitivity
    0.59 pg/mL
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  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    2 - 3 h
    Plate
    Pre-coated
    Protocol
    The microplate provided in this kit has been pre-coated with an antibody specific to Erythropoietin (EPO). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Erythropoietin (EPO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Erythropoietin (EPO) level in the sample or standard.,
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Erythropoietin (EPO) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Erythropoietin (EPO) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all EPO CLIA Kits
    EPO (Erythropoietin (EPO))
    Alternative Name
    Erythropoietin (EPO Products)
    Synonyms
    EPO CLIA Kit, EP CLIA Kit, MVCD2 CLIA Kit, erythropoietin CLIA Kit, erythropoietin S homeolog CLIA Kit, erythropoietin a CLIA Kit, EPO CLIA Kit, epo CLIA Kit, epo.S CLIA Kit, Epo CLIA Kit, epoa CLIA Kit
    Target Type
    Hormone
    Background

    Gene Name: Erythropoietin

    Gene Aliases: EP, Epoetin, Erythropoetin, Hematopoietin, Hemopoietin

    Gene ID
    13856
    UniProt
    P07321
    Pathways
    JAK-STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity
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