Fibrillin 1 CLIA Kit
-
- Target See all Fibrillin 1 (FBN1) CLIA Kits
- Fibrillin 1 (FBN1)
-
Reactivity
- Human
- Detection Method
- Chemiluminescent
- Method Type
- Sandwich ELISA
- Detection Range
- 78.12 pg/mL - 20000 pg/mL
- Minimum Detection Limit
- 78.12 pg/mL
- Application
- ELISA
- Sample Type
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificity
- This assay has high sensitivity and excellent specificity for detection of Fibrillin 1 (FBN1). No significant cross-reactivity or interference between Fibrillin 1 (FBN1) and analogues was observed.
- Sensitivity
- 10.95 pg/mL
- Featured
- Discover our best selling FBN1 ELISA Kit
- Top Product
- Discover our top product FBN1 ELISA Kit
-
-
- Comment
-
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Assay Time
- 2 - 3 h
- Plate
- Pre-coated
- Protocol
- The microplate provided in this kit has been pre-coated with an antibody specific to Fibrillin 1 (FBN1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Fibrillin 1 (FBN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Fibrillin 1 (FBN1) level in the sample or standard.,
- Assay Precision
-
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibrillin 1 (FBN1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibrillin 1 (FBN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
-
- Handling Advice
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Storage
- 4 °C,-20 °C
- Storage Comment
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Expiry Date
- 4-8 months
-
- Target See all Fibrillin 1 (FBN1) CLIA Kits
- Fibrillin 1 (FBN1)
- Alternative Name
- Fibrillin 1 (FBN1 Products)
- Synonyms
- FBN1 CLIA Kit, ACMICD CLIA Kit, ECTOL1 CLIA Kit, FBN CLIA Kit, GPHYSD2 CLIA Kit, MASS CLIA Kit, MFS1 CLIA Kit, OCTD CLIA Kit, SGS CLIA Kit, SSKS CLIA Kit, WMS CLIA Kit, WMS2 CLIA Kit, AI536462 CLIA Kit, B430209H23 CLIA Kit, Fib-1 CLIA Kit, Tsk CLIA Kit, fibrillin 1 CLIA Kit, FBN1 CLIA Kit, Fbn1 CLIA Kit
- Background
-
Gene Name: Fibrillin 1
Gene Aliases: FBN, MASS, MFS1, OCTD, SGS, Asprosin
- Gene ID
- 2200
- UniProt
- P35555
- Pathways
- Maintenance of Protein Location, SARS-CoV-2 Protein Interactome
-