GGH ELISA Kit

Catalog No. ABIN5654503

Product Details GGH ELISA Kit

Target: GGH (gamma-Glutamyl Hydrolase (Conjugase, Folylpolygammaglutamyl Hydrolase) (GGH))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Gamma-Glutamyl Hydrolase (gGH). No significant cross-reactivity or interference between Gamma-Glutamyl Hydrolase (gGH) and analogues was observed.

Sensitivity: 0.056 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gamma-Glutamyl Hydrolase (gGH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Gamma-Glutamyl Hydrolase (gGH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Gamma-Glutamyl Hydrolase (gGH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Gamma-Glutamyl Hydrolase (gGH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gamma-Glutamyl Hydrolase (gGH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gamma-Glutamyl Hydrolase (gGH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for GGH

Target: GGH (gamma-Glutamyl Hydrolase (Conjugase, Folylpolygammaglutamyl Hydrolase) (GGH))

Alternative Name: Gamma-Glutamyl Hydrolase (GGH Products)

Synonyms: CK2A2 ELISA Kit, CSNK2A1 ELISA Kit, GH ELISA Kit, gamma-GH ELISA Kit, zgc:76958 ELISA Kit, wu:fa56a05 ELISA Kit, wu:fj90a01 ELISA Kit, MGC84044 ELISA Kit, GGH ELISA Kit, MGC130662 ELISA Kit, ATGGH2 ELISA Kit, gamma-glutamyl hydrolase 2 ELISA Kit, casein kinase 2 alpha 2 ELISA Kit, gamma-glutamyl hydrolase ELISA Kit, gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase) ELISA Kit, gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase) L homeolog ELISA Kit, gamma-glutamyl hydrolase 2 ELISA Kit, Gamma-glutamyl hydrolase ELISA Kit, CSNK2A2 ELISA Kit, GGH ELISA Kit, Ggh ELISA Kit, ggh ELISA Kit, ggh.L ELISA Kit, ggh.2.L ELISA Kit, GGH2 ELISA Kit, GH ELISA Kit

Background:

Gene Name: Gamma-Glutamyl Hydrolase

Gene Aliases: GH, conjugase, folylpolygammaglutamyl hydrolase, Gamma-Glu-X carboxypeptidase

Gene ID: 8836

UniProt: Q92820

Pathways: SARS-CoV-2 Protein Interactome