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Growth Hormone 1 CLIA Kit

GH1 Reactivity: Human Chemiluminescent Sandwich ELISA 468.75 pg/mL - 30000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Catalog No. ABIN5654893
  • Target See all Growth Hormone 1 (GH1) CLIA Kits
    Growth Hormone 1 (GH1)
    Reactivity
    • 7
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Detection Method
    Chemiluminescent
    Method Type
    Sandwich ELISA
    Detection Range
    468.75 pg/mL - 30000 pg/mL
    Minimum Detection Limit
    468.75 pg/mL
    Application
    ELISA
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Growth Hormone (GH). No significant cross-reactivity or interference between Growth Hormone (GH) and analogues was observed.
    Sensitivity
    17.2 pg/mL
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  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    2 - 3 h
    Plate
    Pre-coated
    Protocol
    The microplate provided in this kit has been pre-coated with an antibody specific to Growth Hormone (GH). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Growth Hormone (GH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Growth Hormone (GH) level in the sample or standard.,
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Hormone (GH) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Hormone (GH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all Growth Hormone 1 (GH1) CLIA Kits
    Growth Hormone 1 (GH1)
    Alternative Name
    Growth Hormone (GH1 Products)
    Synonyms
    GH CLIA Kit, GH-N CLIA Kit, GHN CLIA Kit, IGHD1B CLIA Kit, hGH-N CLIA Kit, Gh1 CLIA Kit, GH1 CLIA Kit, gh CLIA Kit, Gh CLIA Kit, GH2 CLIA Kit, RNGHGP CLIA Kit, ghl CLIA Kit, gh-n CLIA Kit, ghn CLIA Kit, ighd1b CLIA Kit, gh1 CLIA Kit, ghb-A CLIA Kit, GHI CLIA Kit, GHB3 CLIA Kit, growth hormone 1 CLIA Kit, growth hormone CLIA Kit, somatotropin-like CLIA Kit, somatotropin CLIA Kit, growth hormone prepeptide CLIA Kit, growth hormone 1 L homeolog CLIA Kit, growth hormone 1 S homeolog CLIA Kit, Somatotropin-1 CLIA Kit, GH1 CLIA Kit, Gh CLIA Kit, GH CLIA Kit, LOC100305005 CLIA Kit, Gh1 CLIA Kit, LOC100534452 CLIA Kit, LOC100232594 CLIA Kit, LOC100303681 CLIA Kit, gh1 CLIA Kit, LOC100356068 CLIA Kit, LOC100136588 CLIA Kit, gh1.L CLIA Kit, gh1.S CLIA Kit, LOC109081196 CLIA Kit
    Background

    Gene Name: Growth Hormone

    Gene Aliases: GH1, GH-N, GHN, hGH-N, Somatotropin, Hygetropin, Jintropin, Kigtropin, Pituitary Growth Hormone, Growth Hormone, Normal

    Gene ID
    2688
    UniProt
    P01241
    Pathways
    NF-kappaB Signaling, JAK-STAT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Response to Growth Hormone Stimulus, Regulation of Hormone Biosynthetic Process
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