Growth Hormone 1 ELISA Kit

Catalog No. ABIN5654898

Product Details Growth Hormone 1 ELISA Kit

Target: Growth Hormone 1 (GH1)

Reactivity: Pig

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Growth Hormone (GH). No significant cross-reactivity or interference between Growth Hormone (GH) and analogues was observed.

Sensitivity: 0.117 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Hormone (GH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Hormone (GH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Hormone (GH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Growth Hormone (GH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Hormone (GH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Hormone (GH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Growth Hormone 1

Target: Growth Hormone 1 (GH1)

Alternative Name: Growth Hormone (GH1 Products)

Synonyms: GH ELISA Kit, GH-N ELISA Kit, GHN ELISA Kit, IGHD1B ELISA Kit, hGH-N ELISA Kit, Gh1 ELISA Kit, GH1 ELISA Kit, gh ELISA Kit, Gh ELISA Kit, GH2 ELISA Kit, RNGHGP ELISA Kit, ghl ELISA Kit, gh-n ELISA Kit, ghn ELISA Kit, ighd1b ELISA Kit, gh1 ELISA Kit, ghb-A ELISA Kit, GHI ELISA Kit, GHB3 ELISA Kit, growth hormone 1 ELISA Kit, growth hormone ELISA Kit, somatotropin-like ELISA Kit, somatotropin ELISA Kit, growth hormone prepeptide ELISA Kit, growth hormone 1 L homeolog ELISA Kit, growth hormone 1 S homeolog ELISA Kit, Somatotropin-1 ELISA Kit, GH1 ELISA Kit, Gh ELISA Kit, GH ELISA Kit, LOC100305005 ELISA Kit, Gh1 ELISA Kit, LOC100534452 ELISA Kit, LOC100232594 ELISA Kit, LOC100303681 ELISA Kit, gh1 ELISA Kit, LOC100356068 ELISA Kit, LOC100136588 ELISA Kit, gh1.L ELISA Kit, gh1.S ELISA Kit, LOC109081196 ELISA Kit

Background:

Gene Name: Growth Hormone

Gene Aliases: GH1, GH-N, GHN, hGH-N, Somatotropin, Hygetropin, Jintropin, Kigtropin, Pituitary Growth Hormone, Growth Hormone, Normal

Pathways: NF-kappaB Signaling, JAK-STAT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Response to Growth Hormone Stimulus, Regulation of Hormone Biosynthetic Process