GRP78 ELISA Kit

Catalog No. ABIN5654966

Product Details GRP78 ELISA Kit

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Heat Shock 70 kDa Protein 5 (HSPA5). No significant cross-reactivity or interference between Heat Shock 70 kDa Protein 5 (HSPA5) and analogues was observed.

Sensitivity: 0.129 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 70 kDa Protein 5 (HSPA5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock 70 kDa Protein 5 (HSPA5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock 70 kDa Protein 5 (HSPA5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock 70 kDa Protein 5 (HSPA5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock 70kDa Protein 5 (HSPA5) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock 70kDa Protein 5 (HSPA5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for GRP78

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Alternative Name: Heat Shock 70kDa Protein 5 (HSPA5 Products)

Synonyms: GRP78 ELISA Kit, BiP ELISA Kit, GRP-78 ELISA Kit, grp78 ELISA Kit, hspa5a ELISA Kit, BIP ELISA Kit, MIF2 ELISA Kit, AL022860 ELISA Kit, AU019543 ELISA Kit, Bip ELISA Kit, D2Wsu141e ELISA Kit, D2Wsu17e ELISA Kit, Grp78 ELISA Kit, Hsce70 ELISA Kit, SEZ-7 ELISA Kit, Sez7 ELISA Kit, baffled ELISA Kit, mBiP ELISA Kit, cb865 ELISA Kit, fb60h09 ELISA Kit, fi36d04 ELISA Kit, wu:fb60h09 ELISA Kit, wu:fi36d04 ELISA Kit, zgc:55994 ELISA Kit, zgc:77606 ELISA Kit, 78 kDa glucose-regulated protein ELISA Kit, heat shock protein family A (Hsp70) member 5 ELISA Kit, BiP/GRP78 ELISA Kit, glucose-regulated protein 78 ELISA Kit, putative glucose-regulated protein 78 ELISA Kit, Hsp70 family ATPase KAR2 ELISA Kit, heat shock protein family A (Hsp70) member 5 S homeolog ELISA Kit, heat shock 70 kDa protein 5a ELISA Kit, heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) ELISA Kit, heat shock protein 5 ELISA Kit, heat shock protein family A member 5 ELISA Kit, CpipJ_CPIJ003550 ELISA Kit, HSPA5 ELISA Kit, grp78 ELISA Kit, LOC100533358 ELISA Kit, BiP/grp78 ELISA Kit, Tc00.1047053506585.40 ELISA Kit, Tb11.02.5450 ELISA Kit, Tb11.02.5500 ELISA Kit, LMJF_28_1200 ELISA Kit, KAR2 ELISA Kit, LOC100135840 ELISA Kit, hspa5.S ELISA Kit, hspa5 ELISA Kit, Hspa5 ELISA Kit

Background:

Gene Name: Heat Shock 70 kDa Protein 5

Gene Aliases: MIF2, BIP, GRP78, Immunoglobulin heavy chain-binding protein, Glucose Regulated Protein 78, Binding Immunoglobulin Protein, Endoplasmic reticulum lumenal Ca binding grp78

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling