HMOX1 ELISA Kit

Catalog No. ABIN5655013

Product Details HMOX1 ELISA Kit

Target: HMOX1 (Heme Oxygenase (Decycling) 1 (HMOX1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Heme Oxygenase 1, Decycling (HO1). No significant cross-reactivity or interference between Heme Oxygenase 1, Decycling (HO1) and analogues was observed.

Sensitivity: 0.065 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heme Oxygenase 1, Decycling (HO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heme Oxygenase 1, Decycling (HO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heme Oxygenase 1, Decycling (HO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heme Oxygenase 1, Decycling (HO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heme Oxygenase 1, Decycling (HO1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heme Oxygenase 1, Decycling (HO1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for HMOX1

Target: HMOX1 (Heme Oxygenase (Decycling) 1 (HMOX1))

Alternative Name: Heme Oxygenase 1, Decycling (HMOX1 Products)

Synonyms: HMOX1 ELISA Kit, ARABIDOPSIS THALIANA HEME OXYGENASE 1 ELISA Kit, ATHO1 ELISA Kit, F18A8.4 ELISA Kit, F18A8_4 ELISA Kit, GENOMES UNCOUPLED 2 ELISA Kit, GUN2 ELISA Kit, HEME OXYGENASE ELISA Kit, HEME OXYGENASE 1 ELISA Kit, HEME OXYGENASE 6 ELISA Kit, HO1 ELISA Kit, HY1 ELISA Kit, HY6 ELISA Kit, PLASTID HEME OXYGENASE ELISA Kit, REVERSAL OF THE DET PHENOTYPE 4 ELISA Kit, HO-1 ELISA Kit, wu:fc27c04 ELISA Kit, zgc:65984 ELISA Kit, D8Wsu38e ELISA Kit, Hemox ELISA Kit, Hmox ELISA Kit, Hsp32 ELISA Kit, HEOXG ELISA Kit, Heox ELISA Kit, Ho-1 ELISA Kit, Ho1 ELISA Kit, hsp32 ELISA Kit, MGC132176 ELISA Kit, Hmox1 ELISA Kit, HMOX1D ELISA Kit, HSP32 ELISA Kit, bK286B10 ELISA Kit, heme oxygenase 1 ELISA Kit, Plant heme oxygenase (decyclizing) family protein ELISA Kit, Heme oxygenase ELISA Kit, heme oxygenase ELISA Kit, heme oxygenase 1, chloroplastic ELISA Kit, heme oxygenase 1a ELISA Kit, heme oxygenase 1 L homeolog ELISA Kit, HMOX1 ELISA Kit, TED4 ELISA Kit, ho1 ELISA Kit, P9301_RS17555 ELISA Kit, A9601_RS17590 ELISA Kit, MAE_RS06565 ELISA Kit, HO1 ELISA Kit, hmox1a ELISA Kit, CPE0214 ELISA Kit, Cyan7425_2962 ELISA Kit, Hmox1 ELISA Kit, hmox1.L ELISA Kit, AM1_C0205 ELISA Kit, CC1G_11686 ELISA Kit, CpipJ_CPIJ007246 ELISA Kit

Background:

Gene Name: Heme Oxygenase 1, Decycling

Gene Aliases: HMOX1, Hsp32, HMOX1D

UniProt: P06762

Pathways: Transition Metal Ion Homeostasis, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, SARS-CoV-2 Protein Interactome