HNF1A CLIA Kit

Catalog No. ABIN5655087

Product Details HNF1A CLIA Kit

Target: HNF1A (HNF1 Homeobox A (HNF1A))

Reactivity: Mouse

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 156.25 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156.25 pg/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Hepatocyte Nuclear Factor 1 Alpha (HNF1a). No significant cross-reactivity or interference between Hepatocyte Nuclear Factor 1 Alpha (HNF1a) and analogues was observed.

Sensitivity: 5.75 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Hepatocyte Nuclear Factor 1 Alpha (HNF1a). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Hepatocyte Nuclear Factor 1 Alpha (HNF1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Hepatocyte Nuclear Factor 1 Alpha (HNF1a) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hepatocyte Nuclear Factor 1 Alpha (HNF1a) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepatocyte Nuclear Factor 1 Alpha (HNF1a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: HNF1A (HNF1 Homeobox A (HNF1A))

Alternative Name: Hepatocyte Nuclear Factor 1 Alpha (HNF1A Products)

Synonyms: HNF-1A CLIA Kit, HNF1 CLIA Kit, IDDM20 CLIA Kit, LFB1 CLIA Kit, MODY3 CLIA Kit, TCF-1 CLIA Kit, TCF1 CLIA Kit, HNF-1a CLIA Kit, AI323641 CLIA Kit, HNF1-alpha CLIA Kit, HNF1[a] CLIA Kit, Hnf-1 CLIA Kit, Hnf1alpha CLIA Kit, Tcf-1 CLIA Kit, Tcf1 CLIA Kit, HNF-1-alpha CLIA Kit, HNF-1alpha CLIA Kit, LF-B1 CLIA Kit, Lfb1 CLIA Kit, HNF-1 CLIA Kit, HNF1A CLIA Kit, tcf1 CLIA Kit, FB1b CLIA Kit, HNF1b CLIA Kit, XLFB1b1 CLIA Kit, hnf1-B CLIA Kit, lfb1b CLIA Kit, mody3 CLIA Kit, tcf1-B CLIA Kit, tcfb-A CLIA Kit, HNF-1-alpha-A CLIA Kit, HNF-1A-A CLIA Kit, LFB1a1 CLIA Kit, TCF-1-A CLIA Kit, XLFB1 CLIA Kit, XLFB1b CLIA Kit, hnf1-a CLIA Kit, hnf1a CLIA Kit, hnf1a-a CLIA Kit, hnf1a-b CLIA Kit, hnf1a.L CLIA Kit, tcf1-A CLIA Kit, xx:af244934-22s CLIA Kit, HNF1 homeobox A CLIA Kit, HNF1 homeobox A L homeolog CLIA Kit, HNF1 homeobox A S homeolog CLIA Kit, HNF1 homeobox a CLIA Kit, HNF1A CLIA Kit, Hnf1a CLIA Kit, hnf1a CLIA Kit, hnf1a.L CLIA Kit, hnf1a.S CLIA Kit

Background:

Gene Name: Hepatocyte Nuclear Factor 1 Alpha

Gene Aliases: TCF1, HNF1, LFB1, LF-B1, MODY3, Transcription Factor 1,Hepatic, Hepatic Nuclear Factor, Albumin Proximal Factor, Liver-specific transcription factor LF-B1

Pathways: Hormone Transport, Carbohydrate Homeostasis