HNF1A ELISA Kit

Catalog No. ABIN5655088

Product Details HNF1A ELISA Kit

Target: HNF1A (HNF1 Homeobox A (HNF1A))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Hepatocyte Nuclear Factor 1 Alpha (HNF1a). No significant cross-reactivity or interference between Hepatocyte Nuclear Factor 1 Alpha (HNF1a) and analogues was observed.

Sensitivity: 0.058 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hepatocyte Nuclear Factor 1 Alpha (HNF1a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hepatocyte Nuclear Factor 1 Alpha (HNF1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hepatocyte Nuclear Factor 1 Alpha (HNF1a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hepatocyte Nuclear Factor 1 Alpha (HNF1a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hepatocyte Nuclear Factor 1 Alpha (HNF1a) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepatocyte Nuclear Factor 1 Alpha (HNF1a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for HNF1A

Target: HNF1A (HNF1 Homeobox A (HNF1A))

Alternative Name: Hepatocyte Nuclear Factor 1 Alpha (HNF1A Products)

Synonyms: HNF-1A ELISA Kit, HNF1 ELISA Kit, IDDM20 ELISA Kit, LFB1 ELISA Kit, MODY3 ELISA Kit, TCF-1 ELISA Kit, TCF1 ELISA Kit, HNF-1a ELISA Kit, AI323641 ELISA Kit, HNF1-alpha ELISA Kit, HNF1[a] ELISA Kit, Hnf-1 ELISA Kit, Hnf1alpha ELISA Kit, Tcf-1 ELISA Kit, Tcf1 ELISA Kit, HNF-1-alpha ELISA Kit, HNF-1alpha ELISA Kit, LF-B1 ELISA Kit, Lfb1 ELISA Kit, HNF-1 ELISA Kit, HNF1A ELISA Kit, tcf1 ELISA Kit, FB1b ELISA Kit, HNF1b ELISA Kit, XLFB1b1 ELISA Kit, hnf1-B ELISA Kit, lfb1b ELISA Kit, mody3 ELISA Kit, tcf1-B ELISA Kit, tcfb-A ELISA Kit, HNF-1-alpha-A ELISA Kit, HNF-1A-A ELISA Kit, LFB1a1 ELISA Kit, TCF-1-A ELISA Kit, XLFB1 ELISA Kit, XLFB1b ELISA Kit, hnf1-a ELISA Kit, hnf1a ELISA Kit, hnf1a-a ELISA Kit, hnf1a-b ELISA Kit, hnf1a.L ELISA Kit, tcf1-A ELISA Kit, xx:af244934-22s ELISA Kit, HNF1 homeobox A ELISA Kit, HNF1 homeobox A L homeolog ELISA Kit, HNF1 homeobox A S homeolog ELISA Kit, HNF1 homeobox a ELISA Kit, HNF1A ELISA Kit, Hnf1a ELISA Kit, hnf1a ELISA Kit, hnf1a.L ELISA Kit, hnf1a.S ELISA Kit

Background:

Gene Name: Hepatocyte Nuclear Factor 1 Alpha

Gene Aliases: TCF1, HNF1, LFB1, LF-B1, MODY3, Transcription Factor 1,Hepatic, Hepatic Nuclear Factor, Albumin Proximal Factor, Liver-specific transcription factor LF-B1

Pathways: Hormone Transport, Carbohydrate Homeostasis