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C1QBP ELISA Kit

C1QBP Reactivity: Human Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum
Catalog No. ABIN5655205
  • Target See all C1QBP ELISA Kits
    C1QBP (Complement Component 1, Q Subcomponent Binding Protein (C1QBP))
    Reactivity
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    31.25 pg/mL - 2000 pg/mL
    Minimum Detection Limit
    31.25 pg/mL
    Application
    ELISA
    Sample Type
    Plasma, Serum
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Hyaluronan Binding Protein 1 (HABP1). No significant cross-reactivity or interference between Hyaluronan Binding Protein 1 (HABP1) and analogues was observed.
    Sensitivity
    13.8 pg/mL
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  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hyaluronan Binding Protein 1 (HABP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hyaluronan Binding Protein 1 (HABP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hyaluronan Binding Protein 1 (HABP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hyaluronan Binding Protein 1 (HABP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hyaluronan Binding Protein 1 (HABP1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hyaluronan Binding Protein 1 (HABP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all C1QBP ELISA Kits
    C1QBP (Complement Component 1, Q Subcomponent Binding Protein (C1QBP))
    Alternative Name
    Hyaluronan Binding Protein 1 (C1QBP Products)
    Synonyms
    SF2 ELISA Kit, X38k ELISA Kit, gC1qR ELISA Kit, C1QBP ELISA Kit, GC1QBP ELISA Kit, HABP1 ELISA Kit, SF2p32 ELISA Kit, gC1Q-R ELISA Kit, p32 ELISA Kit, AA407365 ELISA Kit, AA986492 ELISA Kit, D11Wsu182e ELISA Kit, P32 ELISA Kit, gC1qBP ELISA Kit, Habp1 ELISA Kit, complement C1q binding protein ELISA Kit, complement component 1, q subcomponent binding protein S homeolog ELISA Kit, complement component 1, q subcomponent binding protein ELISA Kit, C1QBP ELISA Kit, c1qbp.S ELISA Kit, LOC100227158 ELISA Kit, C1qbp ELISA Kit
    Background

    Gene Name: Hyaluronan Binding Protein 1

    Gene Aliases: C1QBP, GC1QBP, SF2p32, GC1Q-R, GC1qR, P32, Complement Component 1 Q Subcomponent Binding Protein, Splicing Factor SF2-Associated Protein, ASF/SF2-associated p32

    Gene ID
    708
    UniProt
    Q07021
    Pathways
    Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
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