HYOU1 ELISA Kit

Catalog No. ABIN5655242

Product Details HYOU1 ELISA Kit

Target: HYOU1 (Hypoxia Up Regulated 1 (HYOU1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Hypoxia Up Regulated 1 (HYOU1). No significant cross-reactivity or interference between Hypoxia Up Regulated 1 (HYOU1) and analogues was observed.

Sensitivity: 0.061 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hypoxia Up Regulated 1 (HYOU1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hypoxia Up Regulated 1 (HYOU1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hypoxia Up Regulated 1 (HYOU1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hypoxia Up Regulated 1 (HYOU1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hypoxia Up Regulated 1 (HYOU1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hypoxia Up Regulated 1 (HYOU1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for HYOU1

Target: HYOU1 (Hypoxia Up Regulated 1 (HYOU1))

Alternative Name: Hypoxia Up Regulated 1 (HYOU1 Products)

Synonyms: GRP-170 ELISA Kit, Grp170 ELISA Kit, HSP12A ELISA Kit, ORP-150 ELISA Kit, ORP150 ELISA Kit, AI415631 ELISA Kit, CBP-140 ELISA Kit, Cab140 ELISA Kit, Orp150 ELISA Kit, id:ibd5056 ELISA Kit, wu:fd14c05 ELISA Kit, zgc:56019 ELISA Kit, HYOU1 ELISA Kit, orp150 ELISA Kit, GRP170 ELISA Kit, hypoxia up-regulated 1 ELISA Kit, hypoxia up-regulated protein 1 ELISA Kit, hypoxia up-regulated 1 S homeolog ELISA Kit, HYOU1 ELISA Kit, Hyou1 ELISA Kit, hyou1 ELISA Kit, LOC100380749 ELISA Kit, hyou1.S ELISA Kit

Background:

Gene Name: Hypoxia Up Regulated 1

Gene Aliases: ORP150, HSP12A, Grp170, Glucose-Regulated Protein 170, 150 kDa oxygen-regulated protein

Gene ID: 10525

UniProt: Q9Y4L1

Pathways: ER-Nucleus Signaling, SARS-CoV-2 Protein Interactome