IGF1R ELISA Kit

Catalog No. ABIN5655404

Product Details IGF1R ELISA Kit

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor 1 Receptor (IGF1R). No significant cross-reactivity or interference between Insulin Like Growth Factor 1 Receptor (IGF1R) and analogues was observed.

Sensitivity: 0.054 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor 1 Receptor (IGF1R). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor 1 Receptor (IGF1R). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor 1 Receptor (IGF1R), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Insulin Like Growth Factor 1 Receptor (IGF1R) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Like Growth Factor 1 Receptor (IGF1R) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Like Growth Factor 1 Receptor (IGF1R) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for IGF1R

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Alternative Name: Insulin Like Growth Factor 1 Receptor (IGF1R Products)

Synonyms: CMT2N ELISA Kit, CD221 ELISA Kit, IGFIR ELISA Kit, IGFR ELISA Kit, JTK13 ELISA Kit, IGFIRC ELISA Kit, IGFR1 ELISA Kit, IGF-1R ELISA Kit, IGF1R ELISA Kit, DKFZp469I0618 ELISA Kit, igf-1r ELISA Kit, xIGF-1R ELISA Kit, xIGFR ELISA Kit, xigf1r ELISA Kit, A330103N21Rik ELISA Kit, D930020L01 ELISA Kit, hyft ELISA Kit, igf-ir ELISA Kit, IGF-IR ELISA Kit, IGF-IRb ELISA Kit, wu:fb03h06 ELISA Kit, IGF-IRa ELISA Kit, sb:eu1090 ELISA Kit, wu:fi20b10 ELISA Kit, alanyl-tRNA synthetase ELISA Kit, insulin like growth factor 1 receptor ELISA Kit, insulin-like growth factor 1 receptor ELISA Kit, insulin-like growth factor 1 ELISA Kit, insulin-like growth factor I receptor ELISA Kit, insulin like growth factor 1 receptor S homeolog ELISA Kit, insulin-like growth factor 1b receptor ELISA Kit, insulin-like growth factor 1a receptor ELISA Kit, AARS ELISA Kit, IGF1R ELISA Kit, Igf1r ELISA Kit, igf1r ELISA Kit, Igf1 ELISA Kit, LOC780503 ELISA Kit, LOC100136427 ELISA Kit, igf1r.S ELISA Kit, igf1rb ELISA Kit, igf1ra ELISA Kit

Background:

Gene Name: Insulin Like Growth Factor 1 Receptor

Gene Aliases: CD221, IGF1-R, IGFIR, JTK13

Pathways: RTK Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagy