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ICAM1 CLIA Kit

ICAM1 Reactivity: Human Chemiluminescent Sandwich ELISA 39.06 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Catalog No. ABIN5655524
  • Target See all ICAM1 CLIA Kits
    ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))
    Reactivity
    • 9
    • 8
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Detection Method
    Chemiluminescent
    Method Type
    Sandwich ELISA
    Detection Range
    39.06 pg/mL - 10000 pg/mL
    Minimum Detection Limit
    39.06 pg/mL
    Application
    ELISA
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Intercellular Adhesion Molecule 1 (ICAM1). No significant cross-reactivity or interference between Intercellular Adhesion Molecule 1 (ICAM1) and analogues was observed.
    Sensitivity
    4.8 pg/mL
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  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    2 - 3 h
    Plate
    Pre-coated
    Protocol
    The microplate provided in this kit has been pre-coated with an antibody specific to Intercellular Adhesion Molecule 1 (ICAM1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Intercellular Adhesion Molecule 1 (ICAM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Intercellular Adhesion Molecule 1 (ICAM1) level in the sample or standard.,
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Intercellular Adhesion Molecule 1 (ICAM1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Intercellular Adhesion Molecule 1 (ICAM1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all ICAM1 CLIA Kits
    ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))
    Alternative Name
    Intercellular Adhesion Molecule 1 (ICAM1 Products)
    Synonyms
    BB2 CLIA Kit, CD54 CLIA Kit, P3.58 CLIA Kit, Icam-1 CLIA Kit, Ly-47 CLIA Kit, MALA-2 CLIA Kit, ICAM CLIA Kit, ICAM-1 CLIA Kit, ICAM1 CLIA Kit, intercellular adhesion molecule 1 CLIA Kit, intercellular adhesion molecule-1 CLIA Kit, ICAM1 CLIA Kit, Icam1 CLIA Kit, ICAM-1 CLIA Kit
    Target Type
    Viral Protein
    Background

    Gene Name: Intercellular Adhesion Molecule 1

    Gene Aliases: CD54, BB2, P3.58, Human Rhinovirus Receptor, Major group rhinovirus receptor

    Gene ID
    3383
    UniProt
    P05362
    Pathways
    Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling
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