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CD25 ELISA Kit

IL2RA Reactivity: Cow Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Plasma, Serum
Catalog No. ABIN5655878
  • Target See all CD25 (IL2RA) ELISA Kits
    CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
    Reactivity
    • 7
    • 5
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Cow
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    15.62 pg/mL - 1000 pg/mL
    Minimum Detection Limit
    15.62 pg/mL
    Application
    ELISA
    Sample Type
    Plasma, Serum
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Interleukin 2 Receptor Alpha (IL2Ra). No significant cross-reactivity or interference between Interleukin 2 Receptor Alpha (IL2Ra) and analogues was observed.
    Sensitivity
    6.3 pg/mL
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  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 2 Receptor Alpha (IL2Ra). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 2 Receptor Alpha (IL2Ra). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 2 Receptor Alpha (IL2Ra), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 2 Receptor Alpha (IL2Ra) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 2 Receptor Alpha (IL2Ra) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 2 Receptor Alpha (IL2Ra) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all CD25 (IL2RA) ELISA Kits
    CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
    Alternative Name
    Interleukin 2 Receptor Alpha (IL2RA Products)
    Synonyms
    CD25 ELISA Kit, IDDM10 ELISA Kit, IL2R ELISA Kit, TCGFR ELISA Kit, IL2RAC ELISA Kit, IL-2RA ELISA Kit, Il2r ELISA Kit, Ly-43 ELISA Kit, interleukin 2 receptor subunit alpha ELISA Kit, interleukin-2 receptor subunit alpha-like ELISA Kit, interleukin 2 receptor, alpha chain ELISA Kit, IL2RA ELISA Kit, Il2ra ELISA Kit, LOC100222957 ELISA Kit
    Background

    Gene Name: Interleukin 2 Receptor Alpha

    Gene Aliases: CD25, IL2R-a, IL2-RA, IDDM10, IL2R, TCGFR, p55, TAC antigen

    Gene ID
    281861
    UniProt
    P12342
    Pathways
    JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
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