LBP ELISA Kit

Catalog No. ABIN5656462

Product Details LBP ELISA Kit

Target: LBP (Lipopolysaccharide Binding Protein (LBP))

Reactivity: Pig

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 7.81 ng/mL - 500 ng/mL

Minimum Detection Limit: 7.81 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Lipopolysaccharide Binding Protein (LBP). No significant cross-reactivity or interference between Lipopolysaccharide Binding Protein (LBP) and analogues was observed.

Sensitivity: 3.5 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipopolysaccharide Binding Protein (LBP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipopolysaccharide Binding Protein (LBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipopolysaccharide Binding Protein (LBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lipopolysaccharide Binding Protein (LBP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lipopolysaccharide Binding Protein (LBP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lipopolysaccharide Binding Protein (LBP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for LBP

Target: LBP (Lipopolysaccharide Binding Protein (LBP))

Alternative Name: Lipopolysaccharide Binding Protein (LBP Products)

Synonyms: BPIFD2 ELISA Kit, Bpifd2 ELISA Kit, Ly88 ELISA Kit, LPSBP ELISA Kit, LBP ELISA Kit, lipopolysaccharide binding protein ELISA Kit, lipopolysaccharide-binding protein ELISA Kit, LBP ELISA Kit, Lbp ELISA Kit, LOC100472839 ELISA Kit

Background:

Gene Name: Lipopolysaccharide Binding Protein

Gene Aliases: LPS-Binding Protein

Gene ID: 397303

UniProt: B3F714

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process