CCL2 CLIA Kit

Catalog No. ABIN5656943

Product Details CCL2 CLIA Kit

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Reactivity: Rabbit

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 625.00 pg/mL - 40000 pg/mL

Minimum Detection Limit: 625.00 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Monocyte Chemotactic Protein 1 (MCP1). No significant cross-reactivity or interference between Monocyte Chemotactic Protein 1 (MCP1) and analogues was observed.

Sensitivity: 21.5 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Monocyte Chemotactic Protein 1 (MCP1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Monocyte Chemotactic Protein 1 (MCP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Monocyte Chemotactic Protein 1 (MCP1) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Monocyte Chemotactic Protein 1 (MCP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Monocyte Chemotactic Protein 1 (MCP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Alternative Name: Monocyte Chemotactic Protein 1 (CCL2 Products)

Synonyms: GDCF-2 CLIA Kit, HC11 CLIA Kit, HSMCR30 CLIA Kit, MCAF CLIA Kit, MCP-1 CLIA Kit, MCP1 CLIA Kit, SCYA2 CLIA Kit, SMC-CF CLIA Kit, AI323594 CLIA Kit, JE CLIA Kit, Scya2 CLIA Kit, Sigje CLIA Kit, MCP-1A CLIA Kit, MCP1A CLIA Kit, C-C motif chemokine ligand 2 CLIA Kit, chemokine (C-C motif) ligand 2 CLIA Kit, C-C motif chemokine 2 CLIA Kit, CCL2 CLIA Kit, Ccl2 CLIA Kit, LOC101120093 CLIA Kit

Background:

Gene Name: Monocyte Chemotactic Protein 1

Gene Aliases: CCL2, GDCF2, HC11, HSMCR30, MCAF, MCP1, SCYA2, SMC-CF, Chemokine C-C-Motif Ligand 2, Monocyte Chemotactic And Activating Factor, Monocyte Secretory Protein JE

Gene ID: 100009130

UniProt: P28292

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection