Paxillin ELISA Kit

Catalog No. ABIN5657651

Product Details Paxillin ELISA Kit

Target: Paxillin (PXN)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Paxillin (Pax). No significant cross-reactivity or interference between Paxillin (Pax) and analogues was observed.

Sensitivity: 0.055 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Paxillin (Pax). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Paxillin (Pax). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Paxillin (Pax), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Paxillin (Pax) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Paxillin (Pax) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Paxillin (Pax) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Paxillin

Target: Paxillin (PXN)

Alternative Name: Paxillin (PXN Products)

Synonyms: AW108311 ELISA Kit, AW123232 ELISA Kit, Pax ELISA Kit, CG18061 ELISA Kit, CG18576 ELISA Kit, CG31794 ELISA Kit, CT40481 ELISA Kit, CT42454 ELISA Kit, DPaxillin ELISA Kit, DPxn ELISA Kit, DPxn37 ELISA Kit, Dmel\\CG31794 ELISA Kit, Dpax ELISA Kit, DpaxA ELISA Kit, PDLP ELISA Kit, dPax ELISA Kit, pax ELISA Kit, wu:fw71f12 ELISA Kit, PXN ELISA Kit, LOC100220858 ELISA Kit, paxillin ELISA Kit, Paxillin ELISA Kit, paxillin S homeolog ELISA Kit, paxillin a ELISA Kit, PXN ELISA Kit, Pxn ELISA Kit, Pax ELISA Kit, pxn.S ELISA Kit, pxna ELISA Kit, LOC696336 ELISA Kit, pxn ELISA Kit

Background:

Gene Name: Paxillin

Gene Aliases: PXN

Gene ID: 5829

UniProt: P49023

Pathways: MAPK Signaling, EGFR Signaling Pathway, Response to Growth Hormone Stimulus, Cell-Cell Junction Organization, Maintenance of Protein Location, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling