PDE4D ELISA Kit

Catalog No. ABIN5657768

Product Details PDE4D ELISA Kit

Target: PDE4D (phosphodiesterase 4D, cAMP-Specific (PDE4D))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Phosphodiesterase 4D, cAMP Specific (PDE4D). No significant cross-reactivity or interference between Phosphodiesterase 4D, cAMP Specific (PDE4D) and analogues was observed.

Sensitivity: 0.112 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphodiesterase 4D, cAMP Specific (PDE4D). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphodiesterase 4D, cAMP Specific (PDE4D). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphodiesterase 4D, cAMP Specific (PDE4D), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphodiesterase 4D, cAMP Specific (PDE4D) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphodiesterase 4D, cAMP Specific (PDE4D) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphodiesterase 4D, cAMP Specific (PDE4D) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for PDE4D

Target: PDE4D (phosphodiesterase 4D, cAMP-Specific (PDE4D))

Alternative Name: Phosphodiesterase 4D, cAMP Specific (PDE4D Products)

Synonyms: ACRDYS2 ELISA Kit, DPDE3 ELISA Kit, HSPDE4D ELISA Kit, PDE43 ELISA Kit, PDE4DN2 ELISA Kit, STRK1 ELISA Kit, CMYA2 ELISA Kit, MMGL ELISA Kit, 9630011N22Rik ELISA Kit, Dpde3 ELISA Kit, 4732458A06Rik ELISA Kit, 9430063L05Rik ELISA Kit, C87016 ELISA Kit, D130016K21Rik ELISA Kit, D3Bwg1078e ELISA Kit, Usmg4 ELISA Kit, mKIAA0454 ELISA Kit, si:dkey-190g11.2 ELISA Kit, phosphodiesterase 4D ELISA Kit, phosphodiesterase 4D interacting protein ELISA Kit, phosphodiesterase 4D, cAMP specific ELISA Kit, phosphodiesterase 4D, cAMP-specific ELISA Kit, phosphodiesterase 4D interacting protein (myomegalin) ELISA Kit, PDE4D ELISA Kit, PDE4DIP ELISA Kit, pde4d ELISA Kit, Pde4dip ELISA Kit, Pde4d ELISA Kit

Background:

Gene Name: Phosphodiesterase 4D, cAMP Specific

Gene Aliases: DPDE3, PDE43, Dunce(Drosophila)Homolog Phosphodiesterase E3, cAMP-specific 3',5'-cyclic phosphodiesterase 4D

Gene ID: 5144

UniProt: Q08499

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling