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PEPCK ELISA Kit

PEPCK Reactivity: Human Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Catalog No. ABIN5657773
  • Target See all PEPCK ELISA Kits
    PEPCK (phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK))
    Reactivity
    • 2
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    1.56 ng/mL - 100 ng/mL
    Minimum Detection Limit
    1.56 ng/mL
    Application
    ELISA
    Sample Type
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Phosphoenolpyruvate Carboxykinase 2, Mitochondrial (PCK2). No significant cross-reactivity or interference between Phosphoenolpyruvate Carboxykinase 2, Mitochondrial (PCK2) and analogues was observed.
    Sensitivity
    0.74 ng/mL
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  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphoenolpyruvate Carboxykinase 2, Mitochondrial (PCK2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphoenolpyruvate Carboxykinase 2, Mitochondrial (PCK2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphoenolpyruvate Carboxykinase 2, Mitochondrial (PCK2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphoenolpyruvate Carboxykinase 2, Mitochondrial (PCK2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphoenolpyruvate Carboxykinase 2, Mitochondrial (PCK2) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphoenolpyruvate Carboxykinase 2, Mitochondrial (PCK2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all PEPCK ELISA Kits
    PEPCK (phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK))
    Alternative Name
    Phosphoenolpyruvate Carboxykinase 2, Mitochondrial (PEPCK Products)
    Synonyms
    PEPCK ELISA Kit, PEPCK-M ELISA Kit, PEPCK2 ELISA Kit, 1810010O14Rik ELISA Kit, 9130022B02Rik ELISA Kit, pck ELISA Kit, zgc:77867 ELISA Kit, id:ibd5157 ELISA Kit, pck2 ELISA Kit, pck2.S ELISA Kit, phosphoenolpyruvate carboxykinase 2, mitochondrial ELISA Kit, phosphoenolpyruvate carboxykinase 2 (mitochondrial) ELISA Kit, phosphoenolpyruvate carboxykinase 2 (mitochondrial) L homeolog ELISA Kit, PCK2 ELISA Kit, Pck2 ELISA Kit, pck2 ELISA Kit, pck2.L ELISA Kit
    Background

    Gene Name: Phosphoenolpyruvate Carboxykinase 2, Mitochondrial

    Gene Aliases: PEPCK, PEPCK2

    Gene ID
    5106
    UniProt
    Q16822
    Pathways
    Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis
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