PLAU CLIA Kit

Catalog No. ABIN5657872

Product Details PLAU CLIA Kit

Target: PLAU (Plasminogen Activator, Urokinase (PLAU))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 31.25 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.25 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Plasminogen Activator, Urokinase (uPA). No significant cross-reactivity or interference between Plasminogen Activator, Urokinase (uPA) and analogues was observed.

Sensitivity: 1.24 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Plasminogen Activator, Urokinase (uPA). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Plasminogen Activator, Urokinase (uPA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Plasminogen Activator, Urokinase (uPA) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Plasminogen Activator, Urokinase (uPA) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Plasminogen Activator, Urokinase (uPA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: PLAU (Plasminogen Activator, Urokinase (PLAU))

Alternative Name: Plasminogen Activator, Urokinase (PLAU Products)

Synonyms: PLAU CLIA Kit, u-PA CLIA Kit, ATF CLIA Kit, BDPLT5 CLIA Kit, QPD CLIA Kit, UPA CLIA Kit, URK CLIA Kit, uPA CLIA Kit, UPAM CLIA Kit, plasminogen activator, urokinase CLIA Kit, urokinase-type plasminogen activator CLIA Kit, PLAU CLIA Kit, CpipJ_CPIJ002131 CLIA Kit, CpipJ_CPIJ006543 CLIA Kit, CpipJ_CPIJ013396 CLIA Kit, CpipJ_CPIJ013623 CLIA Kit, Plau CLIA Kit

Background:

Gene Name: Plasminogen Activator, Urokinase

Gene Aliases: PLAU, ATF, URK, UK, UP-A, Abbokinase, Urokinase-Type Plasminogen Activator

Pathways: Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration