Pleiotrophin ELISA Kit

Catalog No. ABIN5657951

Product Details Pleiotrophin ELISA Kit

Target: Pleiotrophin (PTN)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 7.81 pg/mL - 500 pg/mL

Minimum Detection Limit: 7.81 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Pleiotrophin (PTN). No significant cross-reactivity or interference between Pleiotrophin (PTN) and analogues was observed.

Sensitivity: 3.4 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pleiotrophin (PTN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pleiotrophin (PTN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pleiotrophin (PTN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pleiotrophin (PTN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pleiotrophin (PTN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pleiotrophin (PTN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Pleiotrophin

Target: Pleiotrophin (PTN)

Alternative Name: Pleiotrophin (PTN Products)

Synonyms: zHBNF ELISA Kit, zgc:111787 ELISA Kit, harp ELISA Kit, hbnf ELISA Kit, X-PTF ELISA Kit, hbgf8 ELISA Kit, negf1 ELISA Kit, ptn ELISA Kit, PTN ELISA Kit, HARP ELISA Kit, HBGF8 ELISA Kit, HBNF ELISA Kit, NEGF1 ELISA Kit, PTN-A ELISA Kit, HB-GAM ELISA Kit, HBBM ELISA Kit, HBGF-8 ELISA Kit, OSF-1 ELISA Kit, HBBN ELISA Kit, OSF ELISA Kit, Osf-1 ELISA Kit, Osf1 ELISA Kit, Hbnf ELISA Kit, pleiotrophin ELISA Kit, pleiotrophin L homeolog ELISA Kit, ptn ELISA Kit, PTN ELISA Kit, ptn.L ELISA Kit, Ptn ELISA Kit

Background:

Gene Name: Pleiotrophin

Gene Aliases: HARP, HBGF8, HBNF, NEGF1, Heparin Binding Growth Factor 8, Neurite Growth-Promoting Factor 1, Heparin Affin Regulatory Peptide, Heparin Binding Growth Associated Molecule

Gene ID: 19242

UniProt: P63089

Pathways: RTK Signaling