CD59 ELISA Kit

Catalog No. ABIN5658185

Product Details CD59 ELISA Kit

Target: CD59

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 12.5 pg/mL - 800 pg/mL

Minimum Detection Limit: 12.5 pg/mL

Application: ELISA

Sample Type: Cell Lysate, Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Protectin (CD59). No significant cross-reactivity or interference between Protectin (CD59) and analogues was observed.

Sensitivity: 5.5 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protectin (CD59). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protectin (CD59). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protectin (CD59), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protectin (CD59) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protectin (CD59) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protectin (CD59) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for CD59

Target: CD59

Alternative Name: Protectin (CD59 Products)

Synonyms: 16.3A5 ELISA Kit, 1F5 ELISA Kit, EJ16 ELISA Kit, EJ30 ELISA Kit, EL32 ELISA Kit, G344 ELISA Kit, HRF-20 ELISA Kit, HRF20 ELISA Kit, MAC-IP ELISA Kit, MACIF ELISA Kit, MEM43 ELISA Kit, MIC11 ELISA Kit, MIN1 ELISA Kit, MIN2 ELISA Kit, MIN3 ELISA Kit, MIRL ELISA Kit, MSK21 ELISA Kit, p18-20 ELISA Kit, Cd59a ELISA Kit, Cd59b ELISA Kit, MACIP ELISA Kit, CD59 molecule (CD59 blood group) ELISA Kit, CD59 molecule ELISA Kit, CD59 ELISA Kit, Cd59 ELISA Kit

Background:

Gene Name: Protectin

Gene Aliases: MIC11, MIN1, MIN2, MIN3, MSK21, MAC-IP, MACIF, MIRL, HRF20, Complement Regulatory Protein, 20 kDa homologous restriction factor, Membrane attack complex inhibition factor

Gene ID: 966

UniProt: P13987

Pathways: Complement System