SKP1 ELISA Kit

Catalog No. ABIN5658539

Product Details SKP1 ELISA Kit

Target: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of S-Phase Kinase Associated Protein 1 (SKP1). No significant cross-reactivity or interference between S-Phase Kinase Associated Protein 1 (SKP1) and analogues was observed.

Sensitivity: 0.054 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S-Phase Kinase Associated Protein 1 (SKP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to S-Phase Kinase Associated Protein 1 (SKP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S-Phase Kinase Associated Protein 1 (SKP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of S-Phase Kinase Associated Protein 1 (SKP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level S-Phase Kinase Associated Protein 1 (SKP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level S-Phase Kinase Associated Protein 1 (SKP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for SKP1

Target: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Alternative Name: S-Phase Kinase Associated Protein 1 (SKP1 Products)

Synonyms: EMC19 ELISA Kit, OCP-II ELISA Kit, OCP2 ELISA Kit, SKP1A ELISA Kit, TCEB1L ELISA Kit, p19A ELISA Kit, 15kDa ELISA Kit, 2610043E24Rik ELISA Kit, 2610206H23Rik ELISA Kit, SKP1 ELISA Kit, Tceb1l ELISA Kit, p19Skp1 ELISA Kit, Skp1a ELISA Kit, skp1a ELISA Kit, zgc:73186 ELISA Kit, ocp2 ELISA Kit, emc19 ELISA Kit, ocp-ii ELISA Kit, tceb1l ELISA Kit, ARABIDOPSIS SKP1 HOMOLOGUE 1 ELISA Kit, ASK1 ELISA Kit, ATSKP1 ELISA Kit, S phase kinase-associated protein 1 ELISA Kit, T4O12.17 ELISA Kit, T4O12_17 ELISA Kit, UFO INTERACTING PROTEIN 1 ELISA Kit, UIP1 ELISA Kit, ocp-2 ELISA Kit, S-phase kinase associated protein 1 ELISA Kit, S-phase kinase-associated protein 1 ELISA Kit, S-phase kinase-associated protein 1 pseudogene ELISA Kit, S-phase kinase-associated protein 1A ELISA Kit, S-phase kinase-associated protein 1 S homeolog ELISA Kit, S phase kinase-associated protein 1 ELISA Kit, SKP1 ELISA Kit, skp1 ELISA Kit, LOC100340043 ELISA Kit, Skp1a ELISA Kit, Skp1 ELISA Kit, skp1.S ELISA Kit

Background:

Gene Name: S-Phase Kinase Associated Protein 1

Gene Aliases: SKP1A, EMC19, OCP-II, OCP2, TCEB1L, p19A, Cyclin-A/CDK2-associated p19, RNA polymerase II elongation factor-like protein, Transcription elongation factor B polypeptide 1-like

Gene ID: 6500

UniProt: P63208

Pathways: Cell Division Cycle, Hedgehog Signaling, Mitotic G1-G1/S Phases