SPTBN4 ELISA Kit

Catalog No. ABIN5658904

Product Details SPTBN4 ELISA Kit

Target: SPTBN4 (Spectrin Beta, Non-Erythrocytic 4 (SPTBN4))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Spectrin Beta, Non Erythrocytic 4 (SPTbN4). No significant cross-reactivity or interference between Spectrin Beta, Non Erythrocytic 4 (SPTbN4) and analogues was observed.

Sensitivity: 0.055 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Spectrin Beta, Non Erythrocytic 4 (SPTbN4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Spectrin Beta, Non Erythrocytic 4 (SPTbN4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Spectrin Beta, Non Erythrocytic 4 (SPTbN4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Spectrin Beta, Non Erythrocytic 4 (SPTbN4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Spectrin Beta, Non Erythrocytic 4 (SPTbN4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Spectrin Beta, Non Erythrocytic 4 (SPTbN4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for SPTBN4

Target: SPTBN4 (Spectrin Beta, Non-Erythrocytic 4 (SPTBN4))

Alternative Name: Spectrin Beta, Non Erythrocytic 4 (SPTBN4 Products)

Synonyms: qv ELISA Kit, dyn ELISA Kit, lnd ELISA Kit, SpbIV ELISA Kit, ROSA62 ELISA Kit, nmf261 ELISA Kit, nmf379 ELISA Kit, 1700022P15Rik ELISA Kit, 5830426A08Rik ELISA Kit, Spnb4 ELISA Kit, QV ELISA Kit, SPNB4 ELISA Kit, SPTBN3 ELISA Kit, spectrin beta, non-erythrocytic 4 ELISA Kit, spectrin, beta, non-erythrocytic 4 ELISA Kit, Sptbn4 ELISA Kit, SPTBN4 ELISA Kit

Background:

Gene Name: Spectrin Beta, Non Erythrocytic 4

Gene Aliases: QV, SPNB4, SPTBN3, Beta-IV spectrin, Spectrin, non-erythroid beta chain 3

Gene ID: 57731

UniProt: Q9H254

Pathways: Sensory Perception of Sound, Regulation of Actin Filament Polymerization, Maintenance of Protein Location