Stathmin 1 ELISA Kit
-
- Target See all Stathmin 1 (STMN1) ELISA Kits
- Stathmin 1 (STMN1)
-
Reactivity
- Human, Rat, Mouse
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.156 ng/mL - 10 ng/mL
- Minimum Detection Limit
- 0.156 ng/mL
- Application
- ELISA
- Sample Type
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificity
- This assay has high sensitivity and excellent specificity for detection of Stathmin 1 (STMN1). No significant cross-reactivity or interference between Stathmin 1 (STMN1) and analogues was observed.
- Sensitivity
- 0.058 ng/M
- Featured
- Discover our best selling STMN1 ELISA Kit
- Top Product
- Discover our top product STMN1 ELISA Kit
-
-
- Comment
-
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Assay Time
- 3 h
- Plate
- Pre-coated
- Protocol
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Stathmin 1 (STMN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Stathmin 1 (STMN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Stathmin 1 (STMN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Stathmin 1 (STMN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Assay Precision
-
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Stathmin 1 (STMN1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Stathmin 1 (STMN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
-
- Handling Advice
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Storage
- 4 °C,-20 °C
- Storage Comment
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Expiry Date
- 4-8 months
-
- Target See all Stathmin 1 (STMN1) ELISA Kits
- Stathmin 1 (STMN1)
- Alternative Name
- Stathmin 1 (STMN1 Products)
- Synonyms
- C1orf215 ELISA Kit, LAP18 ELISA Kit, Lag ELISA Kit, OP18 ELISA Kit, PP17 ELISA Kit, PP19 ELISA Kit, PR22 ELISA Kit, SMN ELISA Kit, 19k ELISA Kit, Lap18 ELISA Kit, Op18 ELISA Kit, P18 ELISA Kit, P19 ELISA Kit, Pig ELISA Kit, Pp17 ELISA Kit, Pp18 ELISA Kit, Pp19 ELISA Kit, Pr22 ELISA Kit, Smn ELISA Kit, prosolin ELISA Kit, op18 ELISA Kit, stathmin ELISA Kit, stmn1 ELISA Kit, stmn1a ELISA Kit, lag ELISA Kit, lap18 ELISA Kit, pp17 ELISA Kit, pp19 ELISA Kit, pr22 ELISA Kit, smn ELISA Kit, fj43c10 ELISA Kit, wu:fj38b07 ELISA Kit, wu:fj43c10 ELISA Kit, zgc:110159 ELISA Kit, cb959 ELISA Kit, wu:fb14e04 ELISA Kit, zgc:136942 ELISA Kit, stmn1-a ELISA Kit, stmn1b ELISA Kit, xo35 ELISA Kit, stathmin 1 ELISA Kit, stathmin 1 S homeolog ELISA Kit, stathmin 1b ELISA Kit, stathmin 1a ELISA Kit, stathmin-like ELISA Kit, stathmin ELISA Kit, stathmin 1 L homeolog ELISA Kit, STMN1 ELISA Kit, Stmn1 ELISA Kit, stmn1.S ELISA Kit, stmn1 ELISA Kit, stmn1b ELISA Kit, stmn1a ELISA Kit, LOC100229194 ELISA Kit, stmn1.L ELISA Kit
- Background
-
Gene Name: Stathmin 1
Gene Aliases: LAP18, C1orf215, Lag, OP18, PP17, PP19, PR22, SMN, Metablastin, Prosolin, Oncoprotein 18, Leukemia-associated phosphoprotein p18
- Pathways
- MAPK Signaling, Microtubule Dynamics
-