KIT Ligand CLIA Kit

Catalog No. ABIN5658948

Product Details KIT Ligand CLIA Kit

Target: KIT Ligand (KITLG)

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 31.25 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.25 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Stem Cell Factor (SCF). No significant cross-reactivity or interference between Stem Cell Factor (SCF) and analogues was observed.

Sensitivity: 1.22 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Stem Cell Factor (SCF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Stem Cell Factor (SCF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Stem Cell Factor (SCF) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Stem Cell Factor (SCF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Stem Cell Factor (SCF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: KIT Ligand (KITLG)

Alternative Name: Stem Cell Factor (KITLG Products)

Synonyms: Xkl-1 CLIA Kit, Xsl CLIA Kit, Xsl-1 CLIA Kit, Xsl-2 CLIA Kit, steel CLIA Kit, KITLG CLIA Kit, SCF CLIA Kit, kitl CLIA Kit, kl-1 CLIA Kit, mgf CLIA Kit, scf CLIA Kit, FPH2 CLIA Kit, KL-1 CLIA Kit, Kitl CLIA Kit, MGF CLIA Kit, SF CLIA Kit, SHEP7 CLIA Kit, Clo CLIA Kit, Con CLIA Kit, Gb CLIA Kit, Kitlg CLIA Kit, Mgf CLIA Kit, SLF CLIA Kit, Sl CLIA Kit, blz CLIA Kit, contrasted CLIA Kit, 710-712 CLIA Kit, CSF CLIA Kit, KITL CLIA Kit, KIT ligand L homeolog CLIA Kit, KIT ligand CLIA Kit, kit ligand CLIA Kit, kitlg.L CLIA Kit, KITLG CLIA Kit, kitlg CLIA Kit, Kitl CLIA Kit, Kitlg CLIA Kit

Background:

Gene Name: Stem Cell Factor

Gene Aliases: KITLG, MGF, SF, KL1, Kitl, FPH2, Kit-Ligand, Steel Factor, Mast Cell Growth Factor, Steel factor, Familial Progressive Hyperpigmentation 2

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway