SOD2 ELISA Kit

Catalog No. ABIN5659005

Product Details SOD2 ELISA Kit

Target: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.25 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Superoxide Dismutase 2, Mitochondrial (SOD2). No significant cross-reactivity or interference between Superoxide Dismutase 2, Mitochondrial (SOD2) and analogues was observed.

Sensitivity: 14.5 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Superoxide Dismutase 2, Mitochondrial (SOD2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Superoxide Dismutase 2, Mitochondrial (SOD2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Superoxide Dismutase 2, Mitochondrial (SOD2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Superoxide Dismutase 2, Mitochondrial (SOD2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Superoxide Dismutase 2, Mitochondrial (SOD2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Superoxide Dismutase 2, Mitochondrial (SOD2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for SOD2

Target: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Alternative Name: Superoxide Dismutase 2, Mitochondrial (SOD2 Products)

Synonyms: LOC100101896 ELISA Kit, MNSOD ELISA Kit, GB14346 ELISA Kit, sod2 ELISA Kit, MGC88869 ELISA Kit, Sod2 ELISA Kit, CG8905 ELISA Kit, Dmel\\CG8905 ELISA Kit, Mito SOD ELISA Kit, Mn SOD ELISA Kit, Mn-SOD ELISA Kit, Mn-SOD2 ELISA Kit, MnSOD ELISA Kit, MnSODII ELISA Kit, Mn[2+]SOD ELISA Kit, SOD ELISA Kit, SOD-2 ELISA Kit, SOD2 ELISA Kit, Sod-2 ELISA Kit, dSOD2 ELISA Kit, mitSOD2 ELISA Kit, mnSOD ELISA Kit, IPOB ELISA Kit, MVCD6 ELISA Kit, cb463 ELISA Kit, wu:fj33b01 ELISA Kit, zgc:73051 ELISA Kit, MnSOD ELISA Kit, manganese superoxide dismutase ELISA Kit, superoxide dismutase 2, mitochondrial ELISA Kit, superoxide dismutase 2 ELISA Kit, Mn superoxide dismutase ELISA Kit, Superoxide dismutase 2 (Mn) ELISA Kit, superoxide dismutase 2 L homeolog ELISA Kit, BDBG_07234 ELISA Kit, LOC100101896 ELISA Kit, MNSOD ELISA Kit, Sod2 ELISA Kit, sod2 ELISA Kit, LbMnSOD4 ELISA Kit, SOD2 ELISA Kit, LOC100282741 ELISA Kit, SOD-2 ELISA Kit, sod2.L ELISA Kit

Background:

Gene Name: Superoxide Dismutase 2, Mitochondrial

Gene Aliases: IPO-B, MNSOD, Mn-SOD

UniProt: P07895

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis, Negative Regulation of intrinsic apoptotic Signaling