SOCS3 ELISA Kit

Catalog No. ABIN5659021

Product Details SOCS3 ELISA Kit

Target: SOCS3 (Suppressor of Cytokine Signaling 3 (SOCS3))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Suppressors Of Cytokine Signaling 3 (SOCS3). No significant cross-reactivity or interference between Suppressors Of Cytokine Signaling 3 (SOCS3) and analogues was observed.

Sensitivity: 0.059 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Suppressors Of Cytokine Signaling 3 (SOCS3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Suppressors Of Cytokine Signaling 3 (SOCS3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Suppressors Of Cytokine Signaling 3 (SOCS3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Suppressors Of Cytokine Signaling 3 (SOCS3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Suppressors Of Cytokine Signaling 3 (SOCS3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Suppressors Of Cytokine Signaling 3 (SOCS3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for SOCS3

Target: SOCS3 (Suppressor of Cytokine Signaling 3 (SOCS3))

Alternative Name: Suppressors Of Cytokine Signaling 3 (SOCS3 Products)

Synonyms: socs3 ELISA Kit, fj09e12 ELISA Kit, zgc:56537 ELISA Kit, wu:fj09e12 ELISA Kit, MGC64393 ELISA Kit, MGC75749 ELISA Kit, xsocs3 ELISA Kit, MGC81248 ELISA Kit, atod4 ELISA Kit, cis3 ELISA Kit, cish3 ELISA Kit, socs-3 ELISA Kit, ssi-3 ELISA Kit, ssi3 ELISA Kit, SOCS3 ELISA Kit, ATOD4 ELISA Kit, CIS3 ELISA Kit, Cish3 ELISA Kit, SOCS-3 ELISA Kit, SSI-3 ELISA Kit, SSI3 ELISA Kit, Cis3 ELISA Kit, EF-10 ELISA Kit, Ef10 ELISA Kit, Ssi3 ELISA Kit, Socs-3 ELISA Kit, Ssi-3 ELISA Kit, suppressor of cytokine signaling 3a ELISA Kit, suppressor of cytokine signaling 3 S homeolog ELISA Kit, suppressor of cytokine signaling 3 ELISA Kit, suppressor of cytokine signaling 3 L homeolog ELISA Kit, socs3a ELISA Kit, socs3.S ELISA Kit, socs3 ELISA Kit, socs3.L ELISA Kit, SOCS3 ELISA Kit, Socs3 ELISA Kit

Background:

Gene Name: Suppressors Of Cytokine Signaling 3

Gene Aliases: ATOD4, CIS3, Cish3, MGC71791, SOCS-3, SSI-3, SSI3, Cytokine-inducible SH2 protein 3, STAT-induced STAT inhibitor 3

Gene ID: 89829

UniProt: O88583

Pathways: JAK-STAT Signaling, Response to Growth Hormone Stimulus, Hepatitis C