Synaptophysin ELISA Kit

Catalog No. ABIN5659049

Product Details Synaptophysin ELISA Kit

Target: Synaptophysin (SYP)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.625 ng/mL - 40 ng/mL

Minimum Detection Limit: 0.625 ng/mL

Application: ELISA

Sample Type: Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Synaptophysin (SYP). No significant cross-reactivity or interference between Synaptophysin (SYP) and analogues was observed.

Sensitivity: 0.276 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Synaptophysin (SYP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Synaptophysin (SYP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Synaptophysin (SYP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Synaptophysin (SYP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Synaptophysin (SYP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Synaptophysin (SYP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Synaptophysin

Target: Synaptophysin (SYP)

Alternative Name: Synaptophysin (SYP Products)

Synonyms: syp-B ELISA Kit, xsypii ELISA Kit, MGC86267 ELISA Kit, MRXSYP ELISA Kit, A230093K24Rik ELISA Kit, AI848995 ELISA Kit, Syn ELISA Kit, p38 ELISA Kit, Syp1 ELISA Kit, mrxsyp ELISA Kit, syp ELISA Kit, xsypi ELISA Kit, synaptophysin S homeolog ELISA Kit, synaptophysin ELISA Kit, synaptophysin L homeolog ELISA Kit, synaptophysin a ELISA Kit, syp.S ELISA Kit, SYP ELISA Kit, syp ELISA Kit, Syp ELISA Kit, syp.L ELISA Kit, sypa ELISA Kit

Background:

Gene Name: Synaptophysin

Gene Aliases: Major synaptic vesicle protein p38

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity