TXNIP ELISA Kit

Catalog No. ABIN5659153

Product Details TXNIP ELISA Kit

Target: TXNIP (Thioredoxin Interacting Protein (TXNIP))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Thioredoxin Binding Protein 2 (TBP2). No significant cross-reactivity or interference between Thioredoxin Binding Protein 2 (TBP2) and analogues was observed.

Sensitivity: 0.114 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thioredoxin Binding Protein 2 (TBP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thioredoxin Binding Protein 2 (TBP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thioredoxin Binding Protein 2 (TBP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thioredoxin Binding Protein 2 (TBP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thioredoxin Binding Protein 2 (TBP2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thioredoxin Binding Protein 2 (TBP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for TXNIP

Target: TXNIP (Thioredoxin Interacting Protein (TXNIP))

Alternative Name: Thioredoxin Binding Protein 2 (TXNIP Products)

Synonyms: EST01027 ELISA Kit, HHCPA78 ELISA Kit, THIF ELISA Kit, VDUP1 ELISA Kit, Gm348 ELISA Kit, Trf3 ELISA Kit, TBP2 ELISA Kit, TRF3 ELISA Kit, 1200008J08Rik ELISA Kit, AA682105 ELISA Kit, Hyplip1 ELISA Kit, Tbp-2 ELISA Kit, Vdup1 ELISA Kit, sb:cb368 ELISA Kit, txnip ELISA Kit, thioredoxin interacting protein ELISA Kit, TATA box binding protein like 2 ELISA Kit, TATA-box binding protein like 2 ELISA Kit, thioredoxin interacting protein L homeolog ELISA Kit, thioredoxin interacting protein a ELISA Kit, TXNIP ELISA Kit, Tbpl2 ELISA Kit, TBPL2 ELISA Kit, Txnip ELISA Kit, txnip.L ELISA Kit, txnip ELISA Kit, txnipa ELISA Kit

Background:

Gene Name: Thioredoxin Binding Protein 2

Gene Aliases: TXNIP, HHCPA78, THIF, VDUP1, Thioredoxin Interacting Protein, Vitamin D3-Upregulated Protein 1, Upregulated By 1,25-Dihydroxyvitamin D-3

Gene ID: 56338

UniProt: Q8BG60

Pathways: Protein targeting to Nucleus, Platelet-derived growth Factor Receptor Signaling, Inflammasome