Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

TNFAIP3 ELISA Kit

TNFAIP3 Reactivity: Human Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Catalog No. ABIN5659643
  • Target See all TNFAIP3 ELISA Kits
    TNFAIP3 (Tumor Necrosis Factor, alpha-Induced Protein 3 (TNFAIP3))
    Reactivity
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    78.12 pg/mL - 5000 pg/mL
    Minimum Detection Limit
    78.12 pg/mL
    Application
    ELISA
    Sample Type
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Alpha Induced Protein 3 (TNFaIP3). No significant cross-reactivity or interference between Tumor Necrosis Factor Alpha Induced Protein 3 (TNFaIP3) and analogues was observed.
    Sensitivity
    136 pg/mL
    Featured
    Discover our best selling TNFAIP3 ELISA Kit
    Top Product
    Discover our top product TNFAIP3 ELISA Kit
  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Alpha Induced Protein 3 (TNFaIP3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Alpha Induced Protein 3 (TNFaIP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Alpha Induced Protein 3 (TNFaIP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tumor Necrosis Factor Alpha Induced Protein 3 (TNFaIP3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Necrosis Factor Alpha Induced Protein 3 (TNFaIP3) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Necrosis Factor Alpha Induced Protein 3 (TNFaIP3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all TNFAIP3 ELISA Kits
    TNFAIP3 (Tumor Necrosis Factor, alpha-Induced Protein 3 (TNFAIP3))
    Alternative Name
    Tumor Necrosis Factor Alpha Induced Protein 3 (TNFAIP3 Products)
    Synonyms
    tnfaip3 ELISA Kit, MGC82339 ELISA Kit, TNFAIP3 ELISA Kit, A20 ELISA Kit, OTUD7C ELISA Kit, TNFA1P2 ELISA Kit, Tnfip3 ELISA Kit, TNF alpha induced protein 3 ELISA Kit, TNF alpha induced protein 3 S homeolog ELISA Kit, tumor necrosis factor, alpha-induced protein 3 ELISA Kit, TNFAIP3 ELISA Kit, tnfaip3.S ELISA Kit, Tnfaip3 ELISA Kit
    Background

    Gene Name: Tumor Necrosis Factor Alpha Induced Protein 3

    Gene Aliases: A20, OTUD7C, TNFA1P2, OTU domain-containing protein 7C, Putative DNA-binding protein A20, Zinc finger protein A20

    Gene ID
    7128
    UniProt
    P21580
    Pathways
    TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Production of Molecular Mediator of Immune Response
You are here:
Support