VEGFA CLIA Kit

Catalog No. ABIN5659857

Product Details VEGFA CLIA Kit

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 31.25 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.25 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Vascular Endothelial Growth Factor A (VEGFA). No significant cross-reactivity or interference between Vascular Endothelial Growth Factor A (VEGFA) and analogues was observed.

Sensitivity: 1.17 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Vascular Endothelial Growth Factor A (VEGFA). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Vascular Endothelial Growth Factor A (VEGFA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Vascular Endothelial Growth Factor A (VEGFA) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vascular Endothelial Growth Factor A (VEGFA) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vascular Endothelial Growth Factor A (VEGFA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Alternative Name: Vascular Endothelial Growth Factor A (VEGFA Products)

Synonyms: MVCD1 CLIA Kit, VEGF CLIA Kit, VPF CLIA Kit, Vegf CLIA Kit, Vegf120 CLIA Kit, Vegf164 CLIA Kit, Vegf188 CLIA Kit, Vpf CLIA Kit, vegf CLIA Kit, vegfa CLIA Kit, wu:fj82c06 CLIA Kit, VEGF-A CLIA Kit, VEGF164 CLIA Kit, eVEGF120 CLIA Kit, eVEGF164 CLIA Kit, vegf-a CLIA Kit, vpf CLIA Kit, vefg CLIA Kit, si:dkey-14d8.3 CLIA Kit, wu:fd42e02 CLIA Kit, vascular endothelial growth factor A CLIA Kit, vascular endothelial growth factor Aa CLIA Kit, vascular endothelial growth factor A L homeolog CLIA Kit, vascular endothelial growth factor Ab CLIA Kit, VEGFA CLIA Kit, Vegfa CLIA Kit, vegfaa CLIA Kit, vegfa.L CLIA Kit, vegfa CLIA Kit, vegfab CLIA Kit

Background:

Gene Name: Vascular Endothelial Growth Factor A

Gene Aliases: VEGF, VEGF-A, VPF, Vascular Endothelial Cell Growth Factor A, Vascular Permeability Factor

Gene ID: 7422

UniProt: P15692

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling