ZEB2 ELISA Kit

Catalog No. ABIN5660077

Product Details ZEB2 ELISA Kit

Target: ZEB2 (Zinc Finger E-Box Binding Homeobox 2 (ZEB2))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Zinc Finger Homeobox Protein 1B (ZFHX1B). No significant cross-reactivity or interference between Zinc Finger Homeobox Protein 1B (ZFHX1B) and analogues was observed.

Sensitivity: 0.051 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Zinc Finger Homeobox Protein 1B (ZFHX1B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Zinc Finger Homeobox Protein 1B (ZFHX1B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Zinc Finger Homeobox Protein 1B (ZFHX1B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zinc Finger Homeobox Protein 1B (ZFHX1B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Zinc Finger Homeobox Protein 1B (ZFHX1B) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Zinc Finger Homeobox Protein 1B (ZFHX1B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for ZEB2

Target: ZEB2 (Zinc Finger E-Box Binding Homeobox 2 (ZEB2))

Alternative Name: Zinc Finger Homeobox Protein 1B (ZEB2 Products)

Synonyms: ZFHX1B ELISA Kit, Zfhx1b ELISA Kit, HSPC082 ELISA Kit, SIP-1 ELISA Kit, SIP1 ELISA Kit, SMADIP1 ELISA Kit, 9130203F04Rik ELISA Kit, D130016B08Rik ELISA Kit, Zfx1b ELISA Kit, Zfxh1b ELISA Kit, mKIAA0569 ELISA Kit, XSip1 ELISA Kit, sip1 ELISA Kit, zfhx1b ELISA Kit, si:dkey-52o2.2 ELISA Kit, sip1a ELISA Kit, wu:fc92g08 ELISA Kit, sip1b ELISA Kit, zinc finger E-box binding homeobox 2 ELISA Kit, zinc finger E-box binding homeobox 2 S homeolog ELISA Kit, zinc finger homeobox protein ELISA Kit, zinc finger E-box binding homeobox 2a ELISA Kit, zinc finger E-box binding homeobox 2b ELISA Kit, ZEB2 ELISA Kit, Zeb2 ELISA Kit, zeb2.S ELISA Kit, zeb2 ELISA Kit, zeb2a ELISA Kit, zeb2b ELISA Kit

Background:

Gene Name: Zinc Finger Homeobox Protein 1B

Gene Aliases: ZEB2, SIP1, SIP-1, SMADIP1, SIP1, Zinc Finger E-Box Binding Homeobox 2, SMAD Interacting Protein 1

Gene ID: 24136

UniProt: Q9R0G7

Pathways: Tube Formation