ZEB2 ELISA Kit

Catalog No. ABIN5674882

Product Details ZEB2 ELISA Kit

Target: ZEB2 (Zinc Finger E-Box Binding Homeobox 2 (ZEB2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.25 ng/mL - 80 ng/mL

Minimum Detection Limit: 1.25 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0.6 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for ZEB2

Target: ZEB2 (Zinc Finger E-Box Binding Homeobox 2 (ZEB2))

Alternative Name: Zinc Finger Homeobox Protein 1B (ZFHX1B) (ZEB2 Products)

Synonyms: ZFHX1B ELISA Kit, Zfhx1b ELISA Kit, HSPC082 ELISA Kit, SIP-1 ELISA Kit, SIP1 ELISA Kit, SMADIP1 ELISA Kit, 9130203F04Rik ELISA Kit, D130016B08Rik ELISA Kit, Zfx1b ELISA Kit, Zfxh1b ELISA Kit, mKIAA0569 ELISA Kit, XSip1 ELISA Kit, sip1 ELISA Kit, zfhx1b ELISA Kit, si:dkey-52o2.2 ELISA Kit, sip1a ELISA Kit, wu:fc92g08 ELISA Kit, sip1b ELISA Kit, zinc finger E-box binding homeobox 2 ELISA Kit, zinc finger E-box binding homeobox 2 S homeolog ELISA Kit, zinc finger homeobox protein ELISA Kit, zinc finger E-box binding homeobox 2a ELISA Kit, zinc finger E-box binding homeobox 2b ELISA Kit, ZEB2 ELISA Kit, Zeb2 ELISA Kit, zeb2.S ELISA Kit, zeb2 ELISA Kit, zeb2a ELISA Kit, zeb2b ELISA Kit

Background: Alternative name: ZEB2, SIP1, SIP-1, SMADIP1, SIP1, Zinc Finger E-Box Binding Homeobox 2, SMAD Interacting Protein 1

Gene ID: 9839

UniProt: O60315

Pathways: Tube Formation