DKK3 ELISA Kit
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- Target See all DKK3 ELISA Kits
- DKK3
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Reactivity
- Mouse
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 31.25 pg/mL - 2000 pg/mL
- Minimum Detection Limit
- 31.25 pg/mL
- Application
- ELISA
- Sample Type
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Sensitivity
- 14.3 pg/mL
- Components
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - Material not included
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
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DKK3 ELISA Kit
Reactivity: Mouse Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
DKK3 ELISA KitReactivity: Human Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
DKK3 ELISA KitReactivity: Mouse Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
DKK3 ELISA KitReactivity: Rat Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
DKK3 ELISA KitReactivity: Human AA 22-350 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Assay Time
- 1 - 4.5 h
- Plate
- Pre-coated
- Protocol
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - Restrictions
- For Research Use only
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- Storage
- 4 °C
- Expiry Date
- 12 months
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- Target See all DKK3 ELISA Kits
- DKK3
- Alternative Name
- Dickkopf Related Protein 3 (DKK3) (DKK3 Products)
- Synonyms
- REIC ELISA Kit, RIG ELISA Kit, reic ELISA Kit, dkk-3 ELISA Kit, AW061014 ELISA Kit, C87148 ELISA Kit, Reic ELISA Kit, p29 ELISA Kit, dickkopf WNT signaling pathway inhibitor 3 ELISA Kit, dickkopf WNT signaling pathway inhibitor 3 S homeolog ELISA Kit, DKK3 ELISA Kit, dkk3 ELISA Kit, dkk3.S ELISA Kit, Dkk3 ELISA Kit
- Background
- Alternative name: REIC
- Gene ID
- 50781
- UniProt
- Q9QUN9
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