ALAS1 ELISA Kit

Catalog No. ABIN5674802

Product Details ALAS1 ELISA Kit

Target: ALAS1 (Aminolevulinate, delta-, Synthase 1 (ALAS1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0131 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for ALAS1

Target: ALAS1 (Aminolevulinate, delta-, Synthase 1 (ALAS1))

Alternative Name: Aminolevulinate Delta Synthase 1 (ALAS1) (ALAS1 Products)

Synonyms: ALAS ELISA Kit, ALAS3 ELISA Kit, ALASH ELISA Kit, MIG4 ELISA Kit, ALAS-N ELISA Kit, Alas-1 ELISA Kit, Alas-h ELISA Kit, ALAS-H ELISA Kit, ALASN ELISA Kit, ALAS1 ELISA Kit, wu:fb58d01 ELISA Kit, wu:fi12g09 ELISA Kit, 5'-aminolevulinate synthase 1 ELISA Kit, aminolevulinic acid synthase 1 ELISA Kit, alanyl-tRNA synthetase protein ELISA Kit, aminolevulinate, delta-, synthase 1 ELISA Kit, ALAS1 ELISA Kit, Alas1 ELISA Kit, alas1 ELISA Kit, alas1.S ELISA Kit, alaS1 ELISA Kit

Background: Alternative name: ALAS3, ALAS, ALASH, 5-aminolevulinic acid synthase 1, Delta-ALA synthase 1, 5-aminolevulinate synthase, nonspecific, mitochondrial

Gene ID: 211

UniProt: P13196

Pathways: Regulation of Lipid Metabolism by PPARalpha