ALAS1 ELISA Kit
Rat ALAS1 ELISA Kit, Colorimetric assay for quantification of Rat ALAS1.
Catalog No. ABIN8074435
$898.15
Plus shipping costs $50.00
96 tests
Shipping to:
United States
Delivery in 8 to 13 Business Days
Quick Overview for ALAS1 ELISA Kit (ABIN8074435)
Target
See all ALAS1 ELISA Kits
ALAS1
(Aminolevulinate, delta-, Synthase 1 (ALAS1))
Reactivity
Rat
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
0.781 ng/mL - 50 ng/mL
Application
ELISA
Sample Type
Tissue Homogenate
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Minimum Detection Limit
- 0.781 ng/mL
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Purpose
- Rat Aminolevulinate Delta Synthase 1 (ALAS1) ELISA Kit for detecting ALAS1 in tissue homogenates
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Analytical Method
- Quantitative
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Sensitivity
- 0.36 ng/mL
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Components
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- Pre-coated, ready to use 96-well strip plate
- Standar
- Detection Reagent A
- Detection Reagent B
- TMB Substrate
- Stop Solution
- Assay Diluent A
- Assay Diluent B
- Wash Buffer (30x concentrate)
- Standard Diluent
- Plate Sealer
- Instruction Manual
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Material not included
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- Microplate Reader with 450 nm filter
- Single or multi-channel pipettes with high precision and disposable tips
- Microcentrifuge tubes
- Deionized or distilled watter
- Absorbent paper for blotting the microplate
- Container for wash solution
- 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2
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Aminolevulinate, delta-, Synthase 1 (ALAS1) ELISA Kit
ALAS1 Reactivity: Rat Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
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Sample Volume
- 100 μL
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Assay Time
- 3 h
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Plate
- Pre-coated
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Assay Precision
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Intra-Assay: CV<10%,
Inter-Assay: CV<12% -
Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C for one month, Long term: see manual.
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Expiry Date
- 12 months
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- ALAS1 (Aminolevulinate, delta-, Synthase 1 (ALAS1))
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Alternative Name
- Aminolevulinate Delta Synthase 1
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Background
- Gene Aliases: ALAS3, ALAS, ALASH, 5-aminolevulinic acid synthase 1, Delta-ALA synthase 1, 5-aminolevulinate synthase, nonspecific, mitochondrial
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Gene ID
- 65155
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UniProt
- P13195
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Pathways
- Regulation of Lipid Metabolism by PPARalpha
Target See all ALAS1 ELISA Kits
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