IRAK1 ELISA Kit

Catalog No. ABIN5674831

Product Details IRAK1 ELISA Kit

Target: IRAK1 (Interleukin-1 Receptor-Associated Kinase 1 (IRAK1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0105 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for IRAK1

Target: IRAK1 (Interleukin-1 Receptor-Associated Kinase 1 (IRAK1))

Alternative Name: Interleukin 1 Receptor Associated Kinase 1 (IRAK1) (IRAK1 Products)

Synonyms: IRAK1 ELISA Kit, IRAK ELISA Kit, pelle ELISA Kit, AA408924 ELISA Kit, IRAK-1 ELISA Kit, IRAK1-S ELISA Kit, Il1rak ELISA Kit, Plpk ELISA Kit, mPLK ELISA Kit, RGD1563841 ELISA Kit, interleukin 1 receptor associated kinase 1 ELISA Kit, interleukin-1 receptor-associated kinase 1 ELISA Kit, IRAK1 ELISA Kit, irak1 ELISA Kit, Irak1 ELISA Kit

Background: Alternative name: IRAK1, IRAK, Pelle

Gene ID: 16179

UniProt: Q62406

Pathways: NF-kappaB Signaling, TLR Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades