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NME1 ELISA Kit

NME1 Reactivity: Mouse Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum
Catalog No. ABIN5674914
  • Target See all NME1 ELISA Kits
    NME1 (Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1))
    Reactivity
    • 3
    • 2
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.156 ng/mL - 10 ng/mL
    Minimum Detection Limit
    0.156 ng/mL
    Application
    ELISA
    Sample Type
    Plasma, Serum
    Analytical Method
    Quantitative
    Sensitivity
    0053 ng/mL
    Components
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Material not included
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Assay Time
    1 - 4.5 h
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Storage
    4 °C
    Expiry Date
    12 months
  • Target See all NME1 ELISA Kits
    NME1 (Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1))
    Alternative Name
    Non Metastatic Cells 1, Protein NM23A Expressed In (NME1) (NME1 Products)
    Synonyms
    AWD ELISA Kit, GAAD ELISA Kit, NB ELISA Kit, NBS ELISA Kit, NDKA ELISA Kit, NDPK-A ELISA Kit, NDPKA ELISA Kit, NM23 ELISA Kit, NM23-H1 ELISA Kit, AL024257 ELISA Kit, NM23-M1 ELISA Kit, NM23A ELISA Kit, NDPK-Z1 ELISA Kit, NM23-B ELISA Kit, NM23-Z1 ELISA Kit, ndpkz1 ELISA Kit, nm23b ELISA Kit, nme1 ELISA Kit, nme2 ELISA Kit, nme2b1 ELISA Kit, NME1 ELISA Kit, NME1-NME2 ELISA Kit, NM23-C1 ELISA Kit, ndpk-b ELISA Kit, ndpkb ELISA Kit, nm23-h2 ELISA Kit, nm23ndk ELISA Kit, puf ELISA Kit, NBR-A ELISA Kit, NBR-B ELISA Kit, NME1-1 ELISA Kit, nm23ndk-a ELISA Kit, NDK I ELISA Kit, NDPK I ELISA Kit, T30A10.80 ELISA Kit, T30A10_80 ELISA Kit, NDP kinase I ELISA Kit, NME/NM23 nucleoside diphosphate kinase 1 ELISA Kit, NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 1 ELISA Kit, non-metastatic cells 1, protein (NM23A) expressed in ELISA Kit, NME/NM23 nucleoside diphosphate kinase 2 S homeolog ELISA Kit, NME/NM23 nucleoside diphosphate kinase 2 L homeolog ELISA Kit, nucleoside diphosphate kinase ELISA Kit, nucleoside diphosphate kinase 1 ELISA Kit, NME1 ELISA Kit, Nme1 ELISA Kit, nme2b.1 ELISA Kit, nme2.S ELISA Kit, nme2.L ELISA Kit, LOC547870 ELISA Kit, NDPK1 ELISA Kit, LOC107790563 ELISA Kit
    Background
    Alternative name: AWD, GAAD, NDPKA, NM23-H1, Nucleoside diphosphate kinase A, Granzyme A-activated DNase, Metastasis inhibition factor nm23, Tumor metastatic process-associated protein
    Gene ID
    18102
    UniProt
    P15532
    Pathways
    Apoptosis, Nucleotide Phosphorylation, Carbohydrate Homeostasis, Ribonucleoside Biosynthetic Process
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