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PDE3B ELISA Kit

PDE3B Reactivity: Human Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Catalog No. ABIN5674920
  • Target See all PDE3B products
    PDE3B (phosphodiesterase 3B, CGMP-Inhibited (PDE3B))
    Reactivity
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    78.125 pg/mL - 5000 pg/mL
    Minimum Detection Limit
    78.125 pg/mL
    Application
    ELISA
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Sensitivity
    26.35 pg/mL
    Components
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Material not included
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Assay Time
    1 - 4.5 h
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Storage
    4 °C
    Expiry Date
    12 months
  • Target See all PDE3B products
    PDE3B (phosphodiesterase 3B, CGMP-Inhibited (PDE3B))
    Alternative Name
    Phosphodiesterase 3B, cGMP Inhibited (PDE3B) (PDE3B Products)
    Synonyms
    PDE3B ELISA Kit, HcGIP1 ELISA Kit, cGIPDE1 ELISA Kit, 9830102A01Rik ELISA Kit, AI847709 ELISA Kit, phosphodiesterase 3B ELISA Kit, phosphodiesterase 3B, cGMP-inhibited ELISA Kit, si:ch211-79e4.4 ELISA Kit, phosphodiesterase 3B L homeolog ELISA Kit, PDE3B ELISA Kit, pde3b ELISA Kit, Pde3b ELISA Kit, si:ch211-79e4.4 ELISA Kit, pde3b.L ELISA Kit
    Background
    Alternative name: HcGIP1, cGIPDE1, CGIPDE1, CGIP1, CGI-PDE B, Cyclic GMP-inhibited phosphodiesterase B
    Gene ID
    5140
    UniProt
    Q13370
    Pathways
    Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, cAMP Metabolic Process
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