PPP2R4 ELISA Kit

Catalog No. ABIN5674922

The Human PPP2R4 ELISA Kit (ABIN5674922) is a Colorimetric ELISA Kit designed to quantify Human PPP2R4.

Quick Overview for PPP2R4 ELISA Kit (ABIN5674922)

Target: PPP2R4 (Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4))

Binding Specificity: Regulatory Subunit 4

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Product Details PPP2R4 ELISA Kit

Minimum Detection Limit: 0.156 ng/mL

Analytical Method: Quantitative

Sensitivity: 0070 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for PPP2R4

Target: PPP2R4 (Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4))

Alternative Name: Protein Phosphatase 2A Activator, (PPP2R4)

Background: Alternative name: PP2A, PR53, PTPA, Phosphotyrosyl Phosphatase Activator, PP2A Phosphatase Activator, PP2A, subunit B', PR53 isoform, Serine/threonine-protein phosphatase 2A activator

Gene ID: 5524

UniProt: Q15257

Pathways: PI3K-Akt Signaling, M Phase, Hepatitis C, Toll-Like Receptors Cascades