Presenilin 2 ELISA Kit

Catalog No. ABIN5674924

Product Details Presenilin 2 ELISA Kit

Target: Presenilin 2 (PSEN2) (Presenilin 2 (Alzheimer Disease 4) (PSEN2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0106 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for Presenilin 2

Target: Presenilin 2 (PSEN2) (Presenilin 2 (Alzheimer Disease 4) (PSEN2))

Alternative Name: Presenilin 2 (PS2) (PSEN2 Products)

Synonyms: AD3L ELISA Kit, AD4 ELISA Kit, CMD1V ELISA Kit, PS2 ELISA Kit, STM2 ELISA Kit, ALG-3 ELISA Kit, Ad4h ELISA Kit, Alg3 ELISA Kit, PS-2 ELISA Kit, Psnl2 ELISA Kit, pre2 ELISA Kit, zf-PS2 ELISA Kit, PSNL2 ELISA Kit, PSEN2 ELISA Kit, ad4 ELISA Kit, ps2 ELISA Kit, ad3l ELISA Kit, stm2 ELISA Kit, Ps2 ELISA Kit, presenilin 2 ELISA Kit, presenilin 2 S homeolog ELISA Kit, presenilin 2 (Alzheimer disease 4) ELISA Kit, trefoil factor 1 ELISA Kit, PSEN2 ELISA Kit, Psen2 ELISA Kit, psen2 ELISA Kit, psen2.S ELISA Kit, Tff1 ELISA Kit

Background: Alternative name: PSEN2, AD3L, AD4, PS2, STM2, AD3LP, AD5, Alzheimer Disease 4

Gene ID: 5664

UniProt: P49810

Pathways: Notch Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway