PARP1 ELISA Kit

Catalog No. ABIN5690733

Product Details PARP1 ELISA Kit

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Purpose: Human Cleaved PARP (D214/G215) Cell-based ELISA Kit. This ELISA is for measuring cleaved PARP (Asp-214/Gly-215) in human adherent cell lines.

Sample Type: Adherent Cell Culture

Analytical Method: Semi-Quantitative

Specificity: The antibody pair provided in this kit recognizes human PARP cleaved at sites aspartic acid-214 and glycine-215.

Characteristics:

• Rapidly measure phosphorylated protein in adherent cell lines
• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• No sample lysis is needed
• Compatible with a standard ELISA plate reader

Components:

• Uncoated 96-well Strip Microplate
• Wash Buffers
• Fixing Solution
• Quenching Buffer
• Blocking Buffer
• Anti-phospho antibody
• Anti-pan antibody
• HRP-Conjugated Secondary Antibody
• TMB One-Step Substrate
• Stop Solution

Material not included:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Application Details

Plate: Uncoated

Protocol:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Expiry Date: 6 months

Target Details for PARP1

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Alternative Name: PARP (PARP1 Products)

Synonyms: ADPRT ELISA Kit, ADPRT 1 ELISA Kit, ADPRT1 ELISA Kit, ARTD1 ELISA Kit, PARP ELISA Kit, PARP-1 ELISA Kit, PPOL ELISA Kit, pADPRT-1 ELISA Kit, Adprt ELISA Kit, Parp-1 ELISA Kit, 5830444G22Rik ELISA Kit, AI893648 ELISA Kit, Adprp ELISA Kit, Adprt1 ELISA Kit, C80510 ELISA Kit, parp-1 ELISA Kit, sPARP-1 ELISA Kit, BEST:LD21673 ELISA Kit, CG17685 ELISA Kit, CG17696 ELISA Kit, CG17718 ELISA Kit, CG40411 ELISA Kit, D.PARP ELISA Kit, Dm.pARTa ELISA Kit, Dmel\\CG40411 ELISA Kit, LD21673.3prime ELISA Kit, PARP1 ELISA Kit, dPARP ELISA Kit, parp ELISA Kit, adprt1 ELISA Kit, padprt-1 ELISA Kit, ppol ELISA Kit, si:dkey-206f10.3 ELISA Kit, wu:fc60f12 ELISA Kit, zgc:110092 ELISA Kit, poly(ADP-ribose) polymerase 1 ELISA Kit, poly (ADP-ribose) polymerase 1 ELISA Kit, poly (ADP-ribose) polymerase family, member 1 ELISA Kit, Poly-(ADP-ribose) polymerase ELISA Kit, poly(ADP-ribose) polymerase 1 L homeolog ELISA Kit, PARP1 ELISA Kit, Parp1 ELISA Kit, Parp ELISA Kit, parp1.L ELISA Kit, parp1 ELISA Kit

Gene ID: 142

UniProt: P09874

Pathways: Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location