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TRAF3 ELISA Kit

TRAF3 Reactivity: Human Isoform 2 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Catalog No. ABIN5690963
  • Target See all TRAF3 ELISA Kits
    TRAF3 (TNF Receptor-Associated Factor 3 (TRAF3))
    Binding Specificity
    Isoform 2
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human TRAF-3 Isoform 2 ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair recognizes Human TRAF-3 Isoform 2.
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    4 °C
    Expiry Date
    6 months
  • Target See all TRAF3 ELISA Kits
    TRAF3 (TNF Receptor-Associated Factor 3 (TRAF3))
    Alternative Name
    TRAF-3 (TRAF3 Products)
    Synonyms
    CAP-1 ELISA Kit, CAP1 ELISA Kit, CD40bp ELISA Kit, CRAF1 ELISA Kit, IIAE5 ELISA Kit, LAP1 ELISA Kit, AI528849 ELISA Kit, T-BAM ELISA Kit, amn ELISA Kit, TNF receptor associated factor 3 ELISA Kit, TNF receptor-associated factor 3 ELISA Kit, Tnf receptor-associated factor 3 ELISA Kit, TRAF3 ELISA Kit, Traf3 ELISA Kit
    Pathways
    NF-kappaB Signaling, Apoptosis, TLR Signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades
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