TSC1 ELISA Kit

Catalog No. ABIN5690964

Product Details TSC1 ELISA Kit

Target: TSC1 (Tuberous Sclerosis 1 (TSC1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human TSC1/Hamartin ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human TSC1.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 6 months

Target Details for TSC1

Target: TSC1 (Tuberous Sclerosis 1 (TSC1))

Alternative Name: TSC1 (Hamartin (TSC1 Products)

Synonyms: LAM ELISA Kit, TSC ELISA Kit, hamartin ELISA Kit, mKIAA0243 ELISA Kit, tsc1 ELISA Kit, wu:fc38b04 ELISA Kit, fa99f04 ELISA Kit, wu:fa99f04 ELISA Kit, zgc:63657 ELISA Kit, TSC complex subunit 1 ELISA Kit, tuberous sclerosis 1 ELISA Kit, TSC complex subunit 1b ELISA Kit, TSC complex subunit 1a ELISA Kit, TSC1 ELISA Kit, Tsc1 ELISA Kit, tsc1b ELISA Kit, tsc1 ELISA Kit, tsc1a ELISA Kit

Gene ID: 7248

UniProt: Q92574

Pathways: RTK Signaling, AMPK Signaling, Regulation of Cell Size, Tube Formation