BMP2 ELISA Kit
-
- Target See all BMP2 ELISA Kits
- BMP2 (Bone Morphogenetic Protein 2 (BMP2))
-
Reactivity
- Zebrafish (Danio rerio)
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Purpose
- Zebrafish BMP-2a ELISA Kit.
- Sample Type
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- Specificity
- This ELISA antibody pair recognizes Zebrafish BMP-2a.
- Characteristics
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Components
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Material not included
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling BMP2 ELISA Kit
- Top Product
- Discover our top product BMP2 ELISA Kit
-
-
- Plate
- Pre-coated
- Protocol
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
-
- Storage
- 4 °C
- Expiry Date
- 6 months
-
- Target See all BMP2 ELISA Kits
- BMP2 (Bone Morphogenetic Protein 2 (BMP2))
- Alternative Name
- BMP-2a (BMP2 Products)
- Synonyms
- BDA2 ELISA Kit, BMP2A ELISA Kit, AI467020 ELISA Kit, Bmp2a ELISA Kit, BMP-2 ELISA Kit, xBMP-2 ELISA Kit, xbmp2 ELISA Kit, BMP2 ELISA Kit, bmp2a ELISA Kit, bmp2 ELISA Kit, wu:fc59d09 ELISA Kit, bone morphogenetic protein 2 ELISA Kit, bone morphogenetic protein 2 L homeolog ELISA Kit, Bone morphogenetic protein 2 ELISA Kit, bone morphogenetic protein 2a ELISA Kit, BMP2 ELISA Kit, Bmp2 ELISA Kit, bmp2.L ELISA Kit, bmp2 ELISA Kit, bmp2a ELISA Kit
- Pathways
- Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Growth Factor Binding, Positive Regulation of fat Cell Differentiation
-