TEK ELISA Kit
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- Target See all TEK ELISA Kits
- TEK (TEK Tyrosine Kinase, Endothelial (TEK))
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Reactivity
- Zebrafish (Danio rerio)
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Purpose
- Zebrafish Tie-2 ELISA Kit.
- Sample Type
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- Specificity
- This ELISA antibody pair recognizes Zebrafish Tie-2.
- Characteristics
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Material not included
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our top product TEK ELISA Kit
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- Plate
- Pre-coated
- Protocol
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Storage
- 4 °C
- Expiry Date
- 6 months
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- Target See all TEK ELISA Kits
- TEK (TEK Tyrosine Kinase, Endothelial (TEK))
- Alternative Name
- Tie-2 (TEK Products)
- Synonyms
- TEK ELISA Kit, DKFZp469E064 ELISA Kit, AA517024 ELISA Kit, Cd202b ELISA Kit, Hyk ELISA Kit, Tie2 ELISA Kit, tie-2 ELISA Kit, CD202B ELISA Kit, TIE-2 ELISA Kit, TIE2 ELISA Kit, VMCM ELISA Kit, VMCM1 ELISA Kit, Tie-2 ELISA Kit, tie2 ELISA Kit, TEK receptor tyrosine kinase ELISA Kit, angiopoietin-1 receptor ELISA Kit, TEK tyrosine kinase, endothelial ELISA Kit, TEK ELISA Kit, LOC100408334 ELISA Kit, tek ELISA Kit, Tek ELISA Kit
- Gene ID
- 30747
- UniProt
- O73791
- Pathways
- RTK Signaling, Growth Factor Binding
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