MTOR FLISA Kit
Quick Overview for MTOR FLISA Kit (ABIN5691957)
Target
See all MTOR (mTOR) FLISA KitsBinding Specificity
Reactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- The CytoFluor™ Phospho Cell-Based ELISAs provides a convenient method for high-throughput screening of the effects of various drugs, metabolites and various treatment effects on protein expression and phosphorylation profiles in whole cells.
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Analytical Method
- Qualitative
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Characteristics
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Detection of proteins from pg/mL to ng/mL concentrations
- Convenient and ready-to-use
- High-sensitivity, low background with exceptional signal-to-noise ratio
- 450 nm colorimetric detection
- Human, mouse and rat targets
- For use with cell lysates, supernatants, sera and plasma -
Components
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2 x 96-Well Tissue Culture Microplates
100x Anti-Phospho Target Primary Antibody
100x Anti-Target Primary Antibody
100x Anti-Control Primary Antibody
Dye 1-Conjugated Anti-Rabbit IgG Secondary Antibody
Dye 2-Conjugated Anti-Mouse IgG Secondary Antibody
10x TBS
15x Wash Buffer
Quenching Buffer
Blocking Buffer
Primary Antibody Diluent
SDS Solution
Adhesive Plate Seals
Technical Manual
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Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) ELISA Kit
mTOR Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) ELISA KitmTOR Reactivity: Rat Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) ELISA KitmTOR Reactivity: Mouse Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) ELISA KitmTOR Reactivity: Human Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) ELISA KitmTOR Reactivity: Human Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) ELISA KitmTOR Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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Application Notes
- Detection Range: At least 5000 cells/well
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Assay Time
- 4.5 h
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Plate
- Uncoated
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C/6 Months
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Expiry Date
- 6 months
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- MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))
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Alternative Name
- mTOR
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Gene ID
- 2475
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UniProt
- P42345
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Pathways
- PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, BCR Signaling, Warburg Effect
Target See all MTOR (mTOR) FLISA Kits
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