NF-kB p65 FLISA Kit
Quick Overview for NF-kB p65 FLISA Kit (ABIN5691960)
Target
See all NF-kB p65 (NFkBP65) FLISA KitsBinding Specificity
Reactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- The CytoFluor™ Phospho Cell-Based ELISAs provides a convenient method for high-throughput screening of the effects of various drugs, metabolites and various treatment effects on protein expression and phosphorylation profiles in whole cells.
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Analytical Method
- Qualitative
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Characteristics
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Detection of proteins from pg/mL to ng/mL concentrations
- Convenient and ready-to-use
- High-sensitivity, low background with exceptional signal-to-noise ratio
- 450 nm colorimetric detection
- Human, mouse and rat targets
- For use with cell lysates, supernatants, sera and plasma -
Components
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2 x 96-Well Tissue Culture Microplates
100x Anti-Phospho Target Primary Antibody
100x Anti-Target Primary Antibody
100x Anti-Control Primary Antibody
Dye 1-Conjugated Anti-Rabbit IgG Secondary Antibody
Dye 2-Conjugated Anti-Mouse IgG Secondary Antibody
10x TBS
15x Wash Buffer
Quenching Buffer
Blocking Buffer
Primary Antibody Diluent
SDS Solution
Adhesive Plate Seals
Technical Manual
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Nuclear Factor-kB p65 (NFkBP65) ELISA Kit
NFkBP65 Reactivity: Human Colorimetric DNA-Binding ELISA
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 Reactivity: Rat Colorimetric Sandwich ELISA 78.13 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 Reactivity: Mouse Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 Reactivity: Human Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 Reactivity: Human Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 Reactivity: Rat Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 Reactivity: Mouse Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Nuclear Factor-kB p65 (NFkBP65) ELISA KitNFkBP65 Reactivity: Rat Colorimetric Sandwich ELISA 78.13 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
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Application Notes
- Detection Range: At least 5000 cells/well
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Assay Time
- 4.5 h
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Plate
- Uncoated
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- 4°C/6 Months
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Expiry Date
- 6 months
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- NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
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Alternative Name
- NF-kappaB p65
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Gene ID
- 5970
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UniProt
- Q04206
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Pathways
- NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins
Target See all NF-kB p65 (NFkBP65) FLISA Kits
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