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PI3K p85 alpha/gamma FLISA Kit

PI3K p85a/g Reactivity: Human, Mouse, Rat pTyr199 Fluorometric Cell ELISA Cell Culture Cells
Catalog No. ABIN5691987
  • Target See all PI3K p85 alpha/gamma (PI3K p85a/g) products
    PI3K p85 alpha/gamma (PI3K p85a/g) (Phosphoinositide 3 Kinase, p85 alpha/gamma (PI3K p85a/g))
    Binding Specificity
    pTyr199, pTyr467
    Reactivity
    Human, Mouse, Rat
    Detection Method
    Fluorometric
    Method Type
    Cell ELISA
    Application
    ELISA
    Purpose
    The CytoFluor™ Phospho Cell-Based ELISAs provides a convenient method for high-throughput screening of the effects of various drugs, metabolites and various treatment effects on protein expression and phosphorylation profiles in whole cells.
    Brand
    CytoFluor™
    Sample Type
    Cell Culture Cells
    Analytical Method
    Qualitative
    Characteristics
    Detection of proteins from pg/mL to ng/mL concentrations
    - Convenient and ready-to-use
    - High-sensitivity, low background with exceptional signal-to-noise ratio
    - 450 nm colorimetric detection
    - Human, mouse and rat targets
    - For use with cell lysates, supernatants, sera and plasma
    Components
    2 x 96-Well Tissue Culture Microplates
    100x Anti-Phospho Target Primary Antibody
    100x Anti-Target Primary Antibody
    100x Anti-Control Primary Antibody
    Dye 1-Conjugated Anti-Rabbit IgG Secondary Antibody
    Dye 2-Conjugated Anti-Mouse IgG Secondary Antibody
    10x TBS
    15x Wash Buffer
    Quenching Buffer
    Blocking Buffer
    Primary Antibody Diluent
    SDS Solution
    Adhesive Plate Seals
    Technical Manual
  • Application Notes
    Detection Range: At least 5000 cells/well
    Assay Time
    4.5 h
    Plate
    Uncoated
    Restrictions
    For Research Use only
  • Storage
    4 °C
    Storage Comment
    4°C/6 Months
    Expiry Date
    6 months
  • Target See all PI3K p85 alpha/gamma (PI3K p85a/g) products
    PI3K p85 alpha/gamma (PI3K p85a/g) (Phosphoinositide 3 Kinase, p85 alpha/gamma (PI3K p85a/g))
    Alternative Name
    PI3-kinase p85-alpha/gamma (PI3K p85a/g Products)
    Gene ID
    5295
    UniProt
    P27986, Q92569
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